Structural analysis of the α--D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

S.S. van Leeuwen; S. Kralj; I.H. van Geel-Schutten; G.J. Gerwig; L. Dijkhuizen; J.P. Kamerling

Structural analysis of the α--D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

S.S. van Leeuwen, S. Kralj, I.H. van Geel-Schutten, G.J. Gerwig, L. Dijkhuizen, J.P. Kamerling

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Abstract

The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1→3, 1→6)-α-d-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:

Original language	Undefined/Unknown
Pages (from-to)	1237-1250
Number of pages	14
Journal	Carbohydrate Research
Volume	343
Issue number	7
Publication status	Published - 2008

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Cite this

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title = "Structural analysis of the α--D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme",

abstract = "The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1→3, 1→6)-α-d-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:",

author = "{van Leeuwen}, S.S. and S. Kralj and {van Geel-Schutten}, I.H. and G.J. Gerwig and L. Dijkhuizen and J.P. Kamerling",

year = "2008",

language = "Undefined/Unknown",

volume = "343",

pages = "1237--1250",

journal = "Carbohydrate Research",

issn = "0008-6215",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Structural analysis of the α--D-glucan (EPS180) produced by the Lactobacillus reuteri strain 180 glucansucrase GTF180 enzyme

AU - van Leeuwen, S.S.

AU - Kralj, S.

AU - van Geel-Schutten, I.H.

AU - Gerwig, G.J.

AU - Dijkhuizen, L.

AU - Kamerling, J.P.

PY - 2008

Y1 - 2008

N2 - The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1→3, 1→6)-α-d-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:

AB - The neutral exopolysaccharide EPS180 produced from sucrose by the glucansucrase GTF180 enzyme from Lactobacillus reuteri 180 was found to be a (1→3, 1→6)-α-d-glucan, with no repeating units present. Based on linkage analysis, periodate oxidation, and 1D/2D 1H and 13C NMR spectroscopy of the intact EPS180, as well as MS and NMR analysis of oligosaccharides obtained by partial acid hydrolysis of EPS180, a composite model, that includes all identified structural features, was formulated as follows:

M3 - Article

SN - 0008-6215

VL - 343

SP - 1237

EP - 1250

JO - Carbohydrate Research

JF - Carbohydrate Research

IS - 7

ER -