Stem traits, compartments and tree species affect fungal communities on decaying wood

Shanshan Yang; Lourens Poorter; Eiko E. Kuramae; Ute Sass-Klaassen; Marcio F.A. Leite; Ohana Y.A. Costa; George A. Kowalchuk; Johannes H.C. Cornelissen; Jurgen van Hal; Leo Goudzwaard; Mariet M. Hefting; Richard S.P. van Logtestijn; Frank J. Sterck

doi:10.1111/1462-2920.15953

Stem traits, compartments and tree species affect fungal communities on decaying wood

Shanshan Yang^*, Lourens Poorter, Eiko E. Kuramae, Ute Sass-Klaassen, Marcio F.A. Leite, Ohana Y.A. Costa, George A. Kowalchuk, Johannes H.C. Cornelissen, Jurgen van Hal, Leo Goudzwaard, Mariet M. Hefting, Richard S.P. van Logtestijn, Frank J. Sterck

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Dead wood quantity and quality is important for forest biodiversity, by determining wood-inhabiting fungal assemblages. We therefore evaluated how fungal communities were regulated by stem traits and compartments (i.e. bark, outer- and inner wood) of 14 common temperate tree species. Fresh logs were incubated in a common garden experiment in a forest site in the Netherlands. After 1 and 4 years of decay, the fungal composition of different compartments was assessed using Internal Transcribed Spacer amplicon sequencing. We found that fungal alpha diversity differed significantly across tree species and stem compartments, with bark showing significantly higher fungal diversity than wood. Gymnosperms and Angiosperms hold different fungal communities, and distinct fungi were found between inner wood and other compartments. Stem traits showed significant afterlife effects on fungal communities; traits associated with accessibility (e.g. conduit diameter), stem chemistry (e.g. C, N, lignin) and physical defence (e.g. density) were important factors shaping fungal community structure in decaying stems. Overall, stem traits vary substantially across stem compartments and tree species, thus regulating fungal communities and the long-term carbon dynamics of dead trees.

Original language	English
Pages (from-to)	3625-3639
Number of pages	15
Journal	Environmental Microbiology
Volume	24
Issue number	8
DOIs	https://doi.org/10.1111/1462-2920.15953
Publication status	Published - Aug 2022

Bibliographical note

Funding Information:
The authors thank many participants of the LOGLIFE Team in various years for help with setting up, maintenance and harvesting. The Schovenhorst estate and Staatsbosbeheer Flevoland were very hospitable and helpful in establishing and hosting LOGLIFE throughout the experimental period. The authors thank Agata Pijl and Mattias de Hollander for their technical support in DNA extraction and bioinformatics. Financial support was provided by the Nora Croin Michielsen Fonds and the China Scholarship Council (No. 201706910085). This is publication number 7383 of the Netherlands Institute of Ecology (NIOO‐KNAW).

Funding Information:
The authors thank many participants of the LOGLIFE Team in various years for help with setting up, maintenance and harvesting. The Schovenhorst estate and Staatsbosbeheer Flevoland were very hospitable and helpful in establishing and hosting LOGLIFE throughout the experimental period. The authors thank Agata Pijl and Mattias de Hollander for their technical support in DNA extraction and bioinformatics. Financial support was provided by the Nora Croin Michielsen Fonds and the China Scholarship Council (No. 201706910085). This is publication number 7383 of the Netherlands Institute of Ecology (NIOO-KNAW).

Publisher Copyright:
© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

Funding

The authors thank many participants of the LOGLIFE Team in various years for help with setting up, maintenance and harvesting. The Schovenhorst estate and Staatsbosbeheer Flevoland were very hospitable and helpful in establishing and hosting LOGLIFE throughout the experimental period. The authors thank Agata Pijl and Mattias de Hollander for their technical support in DNA extraction and bioinformatics. Financial support was provided by the Nora Croin Michielsen Fonds and the China Scholarship Council (No. 201706910085). This is publication number 7383 of the Netherlands Institute of Ecology (NIOO‐KNAW).

Keywords

Deadwood
Debris
Decomposition
Diversity
Dynamics
Enzyme-activities
Identification
Physicochemical properties
Quality
Soil

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Environmental Microbiology - 2022 - Yang - Stem traits compartments and tree species affect fungal communities on decayingFinal published version, 7.97 MBLicence: CC BY

Cite this

Yang, S., Poorter, L., Kuramae, E. E., Sass-Klaassen, U., Leite, M. F. A., Costa, O. Y. A., Kowalchuk, G. A., Cornelissen, J. H. C., van Hal, J., Goudzwaard, L., Hefting, M. M., van Logtestijn, R. S. P., & Sterck, F. J. (2022). Stem traits, compartments and tree species affect fungal communities on decaying wood. Environmental Microbiology, 24(8), 3625-3639. https://doi.org/10.1111/1462-2920.15953

@article{10e123fd06ce4b36816f9fd8f628f286,

title = "Stem traits, compartments and tree species affect fungal communities on decaying wood",

abstract = "Dead wood quantity and quality is important for forest biodiversity, by determining wood-inhabiting fungal assemblages. We therefore evaluated how fungal communities were regulated by stem traits and compartments (i.e. bark, outer- and inner wood) of 14 common temperate tree species. Fresh logs were incubated in a common garden experiment in a forest site in the Netherlands. After 1 and 4 years of decay, the fungal composition of different compartments was assessed using Internal Transcribed Spacer amplicon sequencing. We found that fungal alpha diversity differed significantly across tree species and stem compartments, with bark showing significantly higher fungal diversity than wood. Gymnosperms and Angiosperms hold different fungal communities, and distinct fungi were found between inner wood and other compartments. Stem traits showed significant afterlife effects on fungal communities; traits associated with accessibility (e.g. conduit diameter), stem chemistry (e.g. C, N, lignin) and physical defence (e.g. density) were important factors shaping fungal community structure in decaying stems. Overall, stem traits vary substantially across stem compartments and tree species, thus regulating fungal communities and the long-term carbon dynamics of dead trees.",

keywords = "Deadwood, Debris, Decomposition, Diversity, Dynamics, Enzyme-activities, Identification, Physicochemical properties, Quality, Soil",

author = "Shanshan Yang and Lourens Poorter and Kuramae, {Eiko E.} and Ute Sass-Klaassen and Leite, {Marcio F.A.} and Costa, {Ohana Y.A.} and Kowalchuk, {George A.} and Cornelissen, {Johannes H.C.} and {van Hal}, Jurgen and Leo Goudzwaard and Hefting, {Mariet M.} and {van Logtestijn}, {Richard S.P.} and Sterck, {Frank J.}",

note = "Funding Information: The authors thank many participants of the LOGLIFE Team in various years for help with setting up, maintenance and harvesting. The Schovenhorst estate and Staatsbosbeheer Flevoland were very hospitable and helpful in establishing and hosting LOGLIFE throughout the experimental period. The authors thank Agata Pijl and Mattias de Hollander for their technical support in DNA extraction and bioinformatics. Financial support was provided by the Nora Croin Michielsen Fonds and the China Scholarship Council (No. 201706910085). This is publication number 7383 of the Netherlands Institute of Ecology (NIOO‐KNAW). Funding Information: The authors thank many participants of the LOGLIFE Team in various years for help with setting up, maintenance and harvesting. The Schovenhorst estate and Staatsbosbeheer Flevoland were very hospitable and helpful in establishing and hosting LOGLIFE throughout the experimental period. The authors thank Agata Pijl and Mattias de Hollander for their technical support in DNA extraction and bioinformatics. Financial support was provided by the Nora Croin Michielsen Fonds and the China Scholarship Council (No. 201706910085). This is publication number 7383 of the Netherlands Institute of Ecology (NIOO-KNAW). Publisher Copyright: {\textcopyright} 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.",

year = "2022",

month = aug,

doi = "10.1111/1462-2920.15953",

language = "English",

volume = "24",

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journal = "Environmental Microbiology",

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Yang, S, Poorter, L, Kuramae, EE, Sass-Klaassen, U, Leite, MFA, Costa, OYA, Kowalchuk, GA, Cornelissen, JHC, van Hal, J, Goudzwaard, L, Hefting, MM, van Logtestijn, RSP & Sterck, FJ 2022, 'Stem traits, compartments and tree species affect fungal communities on decaying wood', Environmental Microbiology, vol. 24, no. 8, pp. 3625-3639. https://doi.org/10.1111/1462-2920.15953

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AU - Yang, Shanshan

AU - Poorter, Lourens

AU - Kuramae, Eiko E.

AU - Sass-Klaassen, Ute

AU - Leite, Marcio F.A.

AU - Costa, Ohana Y.A.

AU - Kowalchuk, George A.

AU - Cornelissen, Johannes H.C.

AU - van Hal, Jurgen

AU - Goudzwaard, Leo

AU - Hefting, Mariet M.

AU - van Logtestijn, Richard S.P.

AU - Sterck, Frank J.

N1 - Funding Information: The authors thank many participants of the LOGLIFE Team in various years for help with setting up, maintenance and harvesting. The Schovenhorst estate and Staatsbosbeheer Flevoland were very hospitable and helpful in establishing and hosting LOGLIFE throughout the experimental period. The authors thank Agata Pijl and Mattias de Hollander for their technical support in DNA extraction and bioinformatics. Financial support was provided by the Nora Croin Michielsen Fonds and the China Scholarship Council (No. 201706910085). This is publication number 7383 of the Netherlands Institute of Ecology (NIOO‐KNAW). Funding Information: The authors thank many participants of the LOGLIFE Team in various years for help with setting up, maintenance and harvesting. The Schovenhorst estate and Staatsbosbeheer Flevoland were very hospitable and helpful in establishing and hosting LOGLIFE throughout the experimental period. The authors thank Agata Pijl and Mattias de Hollander for their technical support in DNA extraction and bioinformatics. Financial support was provided by the Nora Croin Michielsen Fonds and the China Scholarship Council (No. 201706910085). This is publication number 7383 of the Netherlands Institute of Ecology (NIOO-KNAW). Publisher Copyright: © 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.

PY - 2022/8

Y1 - 2022/8

N2 - Dead wood quantity and quality is important for forest biodiversity, by determining wood-inhabiting fungal assemblages. We therefore evaluated how fungal communities were regulated by stem traits and compartments (i.e. bark, outer- and inner wood) of 14 common temperate tree species. Fresh logs were incubated in a common garden experiment in a forest site in the Netherlands. After 1 and 4 years of decay, the fungal composition of different compartments was assessed using Internal Transcribed Spacer amplicon sequencing. We found that fungal alpha diversity differed significantly across tree species and stem compartments, with bark showing significantly higher fungal diversity than wood. Gymnosperms and Angiosperms hold different fungal communities, and distinct fungi were found between inner wood and other compartments. Stem traits showed significant afterlife effects on fungal communities; traits associated with accessibility (e.g. conduit diameter), stem chemistry (e.g. C, N, lignin) and physical defence (e.g. density) were important factors shaping fungal community structure in decaying stems. Overall, stem traits vary substantially across stem compartments and tree species, thus regulating fungal communities and the long-term carbon dynamics of dead trees.

AB - Dead wood quantity and quality is important for forest biodiversity, by determining wood-inhabiting fungal assemblages. We therefore evaluated how fungal communities were regulated by stem traits and compartments (i.e. bark, outer- and inner wood) of 14 common temperate tree species. Fresh logs were incubated in a common garden experiment in a forest site in the Netherlands. After 1 and 4 years of decay, the fungal composition of different compartments was assessed using Internal Transcribed Spacer amplicon sequencing. We found that fungal alpha diversity differed significantly across tree species and stem compartments, with bark showing significantly higher fungal diversity than wood. Gymnosperms and Angiosperms hold different fungal communities, and distinct fungi were found between inner wood and other compartments. Stem traits showed significant afterlife effects on fungal communities; traits associated with accessibility (e.g. conduit diameter), stem chemistry (e.g. C, N, lignin) and physical defence (e.g. density) were important factors shaping fungal community structure in decaying stems. Overall, stem traits vary substantially across stem compartments and tree species, thus regulating fungal communities and the long-term carbon dynamics of dead trees.

KW - Deadwood

KW - Debris

KW - Decomposition

KW - Diversity

KW - Dynamics

KW - Enzyme-activities

KW - Identification

KW - Physicochemical properties

KW - Quality

KW - Soil

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U2 - 10.1111/1462-2920.15953

DO - 10.1111/1462-2920.15953

M3 - Article

AN - SCOPUS:85125857652

SN - 1462-2912

VL - 24

SP - 3625

EP - 3639

JO - Environmental Microbiology

JF - Environmental Microbiology

IS - 8

ER -

Stem traits, compartments and tree species affect fungal communities on decaying wood

Abstract

Bibliographical note

Funding

Keywords

Access to Document

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