Stable Isotope Tracing Experiments Using LC-MS

Esther A. Zaal*, Jeroen W.A. Jansen, Celia R. Berkers

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Metabolomics has emerged as a pivotal field in understanding cellular function, particularly in the context of disease. In numerous diseases, including cancer, alterations in metabolism play an essential role in disease progression and drug response. Hence, unraveling the metabolic rewiring is of importance to find novel diagnostic and therapeutic strategies. Isotope tracing is a powerful technique for delving deeper into the metabolic wiring of cells. By tracking an isotopically labeled substrate through biochemical reactions in the cell, this technique provides a dynamic understanding of cellular metabolism. This chapter outlines a robust isotope tracing protocol utilizing high-resolution mass spectrometry coupled to liquid chromatography in cell culture-based models. We cover essential aspects of experimental design and analyses, providing a valuable resource for researchers aiming to employ isotopic tracing.

Original languageEnglish
Pages (from-to)103-116
Number of pages14
JournalMethods in molecular biology (Clifton, N.J.)
Volume2855
Early online date2 Oct 2024
DOIs
Publication statusPublished - 2025

Bibliographical note

Publisher Copyright:
© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Fluxomics
  • High-performance liquid chromatography
  • Isotope tracing
  • Mass spectrometry
  • Metabolomics

Fingerprint

Dive into the research topics of 'Stable Isotope Tracing Experiments Using LC-MS'. Together they form a unique fingerprint.

Cite this