Abstract
This contribution will focus exclusively on the total (global) protein composition
(the proteome) of the sperm surface. Immune responses directed towards sperm
surface proteins may cause infertility since functionally intact sperm are under
immune attack. The immune attack can be achieved directly by deteriorating
sperm or by antibody blocking of a sperm surface protein with a specific function
in the fertilization process. Antibodies that bind to the sperm surface proteins
could also impair the fertilization potential of sperm more indirectly by causing
lateral redistribution of the sperm surface proteins and/or by hindrance of the
assemblage of functional membrane protein complexes involved in fertilization.
Currently the information about the sperm plasma membrane proteome is
increasing but has only led to limited understanding of the functionality that is
related to the complex ordering and processing of this specific cell surface. New
proteomic data and new strategies designed for complete coverage of the surface proteome of mammalian sperm will significantly increase our understanding of how fertilization is accomplished but also how immune responses may frustrate this process. This information will become highly relevant for studying immune infertility. An overview is provided about the current knowledge of the spermsurface and how this structure should experimentally be approached for proteomic studies. Comparative analysis of different mammalian species is covered as this will provide better understanding for the possibilities and limitations of analyzing the surface proteome of human sperm.
(the proteome) of the sperm surface. Immune responses directed towards sperm
surface proteins may cause infertility since functionally intact sperm are under
immune attack. The immune attack can be achieved directly by deteriorating
sperm or by antibody blocking of a sperm surface protein with a specific function
in the fertilization process. Antibodies that bind to the sperm surface proteins
could also impair the fertilization potential of sperm more indirectly by causing
lateral redistribution of the sperm surface proteins and/or by hindrance of the
assemblage of functional membrane protein complexes involved in fertilization.
Currently the information about the sperm plasma membrane proteome is
increasing but has only led to limited understanding of the functionality that is
related to the complex ordering and processing of this specific cell surface. New
proteomic data and new strategies designed for complete coverage of the surface proteome of mammalian sperm will significantly increase our understanding of how fertilization is accomplished but also how immune responses may frustrate this process. This information will become highly relevant for studying immune infertility. An overview is provided about the current knowledge of the spermsurface and how this structure should experimentally be approached for proteomic studies. Comparative analysis of different mammalian species is covered as this will provide better understanding for the possibilities and limitations of analyzing the surface proteome of human sperm.
| Original language | English |
|---|---|
| Title of host publication | Immune infertility 2nd edition |
| Editors | Walter K.H. Krauze, Rajesh K. Naz |
| Place of Publication | Cham, Switzerland |
| Publisher | Springer |
| Pages | 49-76 |
| Number of pages | 27 |
| Edition | 2 |
| ISBN (Electronic) | 978-3-319-40788-3 |
| ISBN (Print) | 978-3-319-40786-9 |
| DOIs | |
| Publication status | Published - 25 Jan 2017 |
Keywords
- Sugar
- Titanium
- Dioxide
- Albumin
- Lipase