Specific serology for emerging human coronaviruses by protein microarray

C Reusken; H Mou; G J Godeke; L van der Hoek; B Meyer; M A Müller; B Haagmans; R de Sousa; Nancy Schuurman; U Dittmer; P Rottier; A Osterhaus; C Drosten; B J Bosch; M Koopmans

doi:10.2807/1560-7917.ES2013.18.14.20441

Specific serology for emerging human coronaviruses by protein microarray

C Reusken, H Mou, G J Godeke, L van der Hoek, B Meyer, M A Müller, B Haagmans, R de Sousa, Nancy Schuurman, U Dittmer, P Rottier, A Osterhaus, C Drosten, B J Bosch, M Koopmans

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

Original language	English
Article number	20441
Journal	Eurosurveillance
Volume	18
Issue number	14
DOIs	https://doi.org/10.2807/1560-7917.ES2013.18.14.20441
Publication status	Published - 2013

Keywords

Coronavirus
Coronavirus Infections
Female
Humans
Male
Protein Array Analysis
Sequence Homology, Amino Acid

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Reusken, C., Mou, H., Godeke, G. J., van der Hoek, L., Meyer, B., Müller, M. A., Haagmans, B., de Sousa, R., Schuurman, N., Dittmer, U., Rottier, P., Osterhaus, A., Drosten, C., Bosch, B. J., & Koopmans, M. (2013). Specific serology for emerging human coronaviruses by protein microarray. Eurosurveillance, 18(14), Article 20441. https://doi.org/10.2807/1560-7917.ES2013.18.14.20441

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AU - Reusken, C

AU - Mou, H

AU - Godeke, G J

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AU - Meyer, B

AU - Müller, M A

AU - Haagmans, B

AU - de Sousa, R

AU - Schuurman, Nancy

AU - Dittmer, U

AU - Rottier, P

AU - Osterhaus, A

AU - Drosten, C

AU - Bosch, B J

AU - Koopmans, M

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KW - Coronavirus

KW - Coronavirus Infections

KW - Female

KW - Humans

KW - Male

KW - Protein Array Analysis

KW - Sequence Homology, Amino Acid

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ER -

Specific serology for emerging human coronaviruses by protein microarray

Abstract

Keywords

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