Specific proteome changes in platelets from individuals with GATA1-, GFI1B-, and RUNX1-linked bleeding disorders

M G J M Van Bergen, A E Marneth, A J Hoogendijk, F P J Van Alphen, E Van den Akker, B A P Laros-Van Gorkom, M Hoeks, A Simons, S A De Munnik, J J W M Janssen, J H A Martens, J H Jansen, A B Meijer, B A Van der Reijden

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Mutations in the transcription factors GATA binding factor 1 (GATA1), growth factor independence 1B (GFI1B), and Runt-related transcription factor 1 (RUNX1) cause familial platelet and bleeding disorders. Mutant platelets exhibit common abnormalities including an α-granule reduction resulting in a grayish appearance in blood smears. This suggests that similar pathways are deregulated by different transcription factor mutations. To identify common factors, full platelet proteomes from 11 individuals with mutant GATA1R216Q, GFI1BQ287*, RUNX1Q154Rfs, or RUNX1TD2-6 and 28 healthy controls were examined by label-free quantitative mass spectrometry. In total, 2875 platelet proteins were reliably quantified. Clustering analysis of more than 300 differentially expressed proteins revealed profound differences between cases and controls. Among cases, 44 of 143 significantly downregulated proteins were assigned to platelet function, hemostasis, and granule biology, in line with platelet dysfunction and bleedings. Remarkably, none of these proteins were significantly diminished in all affected cases. Similarly, no proteins were commonly overrepresented in all affected cases compared with controls. These data indicate that the studied transcription factor mutations alter platelet proteomes in distinct largely nonoverlapping manners. This work provides the quantitative landscape of proteins that affect platelet function when deregulated by mutated transcription factors in inherited bleeding disorders.

Original languageEnglish
Pages (from-to)86-90
Number of pages5
JournalBlood
Volume138
Issue number1
DOIs
Publication statusPublished - 8 Jul 2021
Externally publishedYes

Keywords

  • Blood Platelet Disorders/metabolism
  • Blood Platelets/metabolism
  • Core Binding Factor Alpha 2 Subunit/metabolism
  • GATA1 Transcription Factor/metabolism
  • Homeostasis
  • Humans
  • Mutation/genetics
  • Proteome/metabolism
  • Proto-Oncogene Proteins/metabolism
  • Repressor Proteins/metabolism
  • Signal Transduction
  • Transcription Factors/genetics

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