Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

Matt Timmers; Wouter Peeters; Niels J Hauwert; Cristianne J F Rijcken; Tina Vermonden; Ingrid Dijkgraaf; Rob M J Liskamp

doi:10.1039/d3cc03397j

Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

Matt Timmers
, Wouter Peeters
, Niels J Hauwert
, Cristianne J F Rijcken
, Tina Vermonden
, Ingrid Dijkgraaf
, Rob M J Liskamp

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc.

Original language	English
Pages (from-to)	11397-11400
Number of pages	4
Journal	Chemical communications (Cambridge, England)
Volume	59
Issue number	76
DOIs	https://doi.org/10.1039/d3cc03397j
Publication status	Published - 28 Sept 2023

Bibliographical note

Publisher Copyright:
© 2023 The Royal Society of Chemistry.

Keywords

Azides
Cycloaddition Reaction
Peptides
Antibodies
Alkynes

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title = "Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition",

abstract = "The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc. ",

keywords = "Azides, Cycloaddition Reaction, Peptides, Antibodies, Alkynes",

author = "Matt Timmers and Wouter Peeters and Hauwert, \{Niels J\} and Rijcken, \{Cristianne J F\} and Tina Vermonden and Ingrid Dijkgraaf and Liskamp, \{Rob M J\}",

note = "Publisher Copyright: {\textcopyright} 2023 The Royal Society of Chemistry.",

year = "2023",

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doi = "10.1039/d3cc03397j",

language = "English",

volume = "59",

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T1 - Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

AU - Timmers, Matt

AU - Peeters, Wouter

AU - Hauwert, Niels J

AU - Rijcken, Cristianne J F

AU - Vermonden, Tina

AU - Dijkgraaf, Ingrid

AU - Liskamp, Rob M J

PY - 2023/9/28

Y1 - 2023/9/28

N2 - The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc.

AB - The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc.

KW - Azides

KW - Cycloaddition Reaction

KW - Peptides

KW - Antibodies

KW - Alkynes

UR - https://www.scopus.com/pages/publications/85171646705

U2 - 10.1039/d3cc03397j

DO - 10.1039/d3cc03397j

M3 - Article

C2 - 37668179

SN - 1359-7345

VL - 59

SP - 11397

EP - 11400

JO - Chemical communications (Cambridge, England)

JF - Chemical communications (Cambridge, England)

IS - 76

ER -

Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

Abstract

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Keywords

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