Specific N-terminal attachment of TMTHSI linkers to native peptides and proteins for strain-promoted azide alkyne cycloaddition

Matt Timmers, Wouter Peeters, Niels J Hauwert, Cristianne J F Rijcken, Tina Vermonden, Ingrid Dijkgraaf, Rob M J Liskamp

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The site specific attachment of the reactive TMTHSI-click handle to the N-terminus of peptides and proteins is described. The resulting molecular constructs can be used in strain-promoted azide alkyne cycloaddition (SPAAC) for reaction with azide containing proteins e.g., antibodies, peptides, nanoparticles, fluorescent dyes, chelators for radioactive isotopes and SPR-chips etc.

Original languageEnglish
Pages (from-to)11397-11400
Number of pages4
JournalChemical communications (Cambridge, England)
Volume59
Issue number76
DOIs
Publication statusPublished - 28 Sept 2023

Bibliographical note

Publisher Copyright:
© 2023 The Royal Society of Chemistry.

Keywords

  • Azides
  • Cycloaddition Reaction
  • Peptides
  • Antibodies
  • Alkynes

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