Speciation of Arsenic Compounds in Urine by LC-ICP MS

Rob Ritsema; Laure Dukan; Toni Roig I Navarro; Walter Van Leeuwen; Nanda Oliveira; Pierre Wolfs; Erik Lebret

doi:10.1002/(SICI)1099-0739(199808/09)12:8/9<591::AID-AOC767>3.0.CO;2-E

Speciation of Arsenic Compounds in Urine by LC-ICP MS

Rob Ritsema^*
, Laure Dukan
, Toni Roig I Navarro
, Walter Van Leeuwen
, Nanda Oliveira
, Pierre Wolfs
, Erik Lebret

^*Corresponding author for this work

IRAS OH Epidemiology Microbial Agents

National Institute of Public Health and the Environment
Jaume I University

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The method validation for the speciation of five arsenic species in urine samples by liquid chromatography hyphenated to inductively coupled plasma mass spectrometry is described. Arsenic species which are identified and quantified in urine samples are the anions of arsenic(III), arsenic(V), monomethylarsonic acid and dimethylarsinic acid, and the cationic arsenobetaine. Detection limits were obtained in the range 03-0.4 (μg As 1^-1 while the repeatability was in the range 3-4% (RSD) for concentrations above five times the detection limit. Urine samples could be analysed directly after a tenfold dilution step. Arsenic compound concentrations were determined in urine samples from a volunteer who consumed a portion of tuna fish high in arsenobetaine. It was found that arsenobetaine was excreted rapidly via the urine with maximum concentrations after 12 h. Nearly complete arsenobetaine excretion was reached after 48 h. Background levels of arsenic compounds were determined in 61 urine samples from non-exposed inhabitants of The Netherlands.

Original language	English
Pages (from-to)	591-599
Number of pages	9
Journal	Applied Organometallic Chemistry
Volume	12
Issue number	8-9
DOIs	https://doi.org/10.1002/(SICI)1099-0739(199808/09)12:8/9<591::AID-AOC767>3.0.CO;2-E
Publication status	Published - 1998

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Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Keywords

Arsenic speciation
Background levels
LC-ICP-MS
The Netherlands
Urine samples
Validation

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10.1002/(SICI)1099-0739(199808/09)12:8/9<591::AID-AOC767>3.0.CO;2-E

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title = "Speciation of Arsenic Compounds in Urine by LC-ICP MS",

abstract = "The method validation for the speciation of five arsenic species in urine samples by liquid chromatography hyphenated to inductively coupled plasma mass spectrometry is described. Arsenic species which are identified and quantified in urine samples are the anions of arsenic(III), arsenic(V), monomethylarsonic acid and dimethylarsinic acid, and the cationic arsenobetaine. Detection limits were obtained in the range 03-0.4 (μg As 1-1 while the repeatability was in the range 3-4\% (RSD) for concentrations above five times the detection limit. Urine samples could be analysed directly after a tenfold dilution step. Arsenic compound concentrations were determined in urine samples from a volunteer who consumed a portion of tuna fish high in arsenobetaine. It was found that arsenobetaine was excreted rapidly via the urine with maximum concentrations after 12 h. Nearly complete arsenobetaine excretion was reached after 48 h. Background levels of arsenic compounds were determined in 61 urine samples from non-exposed inhabitants of The Netherlands.",

keywords = "Arsenic speciation, Background levels, LC-ICP-MS, The Netherlands, Urine samples, Validation",

author = "Rob Ritsema and Laure Dukan and \{Roig I Navarro\}, Toni and \{Van Leeuwen\}, Walter and Nanda Oliveira and Pierre Wolfs and Erik Lebret",

year = "1998",

doi = "10.1002/(SICI)1099-0739(199808/09)12:8/9<591::AID-AOC767>3.0.CO;2-E",

language = "English",

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T1 - Speciation of Arsenic Compounds in Urine by LC-ICP MS

AU - Ritsema, Rob

AU - Dukan, Laure

AU - Roig I Navarro, Toni

AU - Van Leeuwen, Walter

AU - Oliveira, Nanda

AU - Wolfs, Pierre

AU - Lebret, Erik

PY - 1998

Y1 - 1998

N2 - The method validation for the speciation of five arsenic species in urine samples by liquid chromatography hyphenated to inductively coupled plasma mass spectrometry is described. Arsenic species which are identified and quantified in urine samples are the anions of arsenic(III), arsenic(V), monomethylarsonic acid and dimethylarsinic acid, and the cationic arsenobetaine. Detection limits were obtained in the range 03-0.4 (μg As 1-1 while the repeatability was in the range 3-4% (RSD) for concentrations above five times the detection limit. Urine samples could be analysed directly after a tenfold dilution step. Arsenic compound concentrations were determined in urine samples from a volunteer who consumed a portion of tuna fish high in arsenobetaine. It was found that arsenobetaine was excreted rapidly via the urine with maximum concentrations after 12 h. Nearly complete arsenobetaine excretion was reached after 48 h. Background levels of arsenic compounds were determined in 61 urine samples from non-exposed inhabitants of The Netherlands.

AB - The method validation for the speciation of five arsenic species in urine samples by liquid chromatography hyphenated to inductively coupled plasma mass spectrometry is described. Arsenic species which are identified and quantified in urine samples are the anions of arsenic(III), arsenic(V), monomethylarsonic acid and dimethylarsinic acid, and the cationic arsenobetaine. Detection limits were obtained in the range 03-0.4 (μg As 1-1 while the repeatability was in the range 3-4% (RSD) for concentrations above five times the detection limit. Urine samples could be analysed directly after a tenfold dilution step. Arsenic compound concentrations were determined in urine samples from a volunteer who consumed a portion of tuna fish high in arsenobetaine. It was found that arsenobetaine was excreted rapidly via the urine with maximum concentrations after 12 h. Nearly complete arsenobetaine excretion was reached after 48 h. Background levels of arsenic compounds were determined in 61 urine samples from non-exposed inhabitants of The Netherlands.

KW - Arsenic speciation

KW - Background levels

KW - LC-ICP-MS

KW - The Netherlands

KW - Urine samples

KW - Validation

UR - https://www.scopus.com/pages/publications/0000835382

U2 - 10.1002/(SICI)1099-0739(199808/09)12:8/9<591::AID-AOC767>3.0.CO;2-E

DO - 10.1002/(SICI)1099-0739(199808/09)12:8/9<591::AID-AOC767>3.0.CO;2-E

M3 - Article

AN - SCOPUS:0000835382

SN - 0268-2605

VL - 12

SP - 591

EP - 599

JO - Applied Organometallic Chemistry

JF - Applied Organometallic Chemistry

IS - 8-9

ER -

Speciation of Arsenic Compounds in Urine by LC-ICP MS

Abstract

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Keywords

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