Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice

Huawei Su; Gooitzen M van Dam; Carlijn I Buis; Dorien S Visser; Jan Willem Hesselink; Theo A Schuurs; Henri G D Leuvenink; Christopher H. Contag; Robert J Porte

doi:10.1002/lt.20852

Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice

Huawei Su, Gooitzen M van Dam, Carlijn I Buis, Dorien S Visser, Jan Willem Hesselink, Theo A Schuurs, Henri G D Leuvenink, Christopher H. Contag, Robert J Porte

University Medical Center Groningen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Upregulation of heme oxygenase-1 (HO-1) has been proposed as a critical mechanism protecting against cellular stress during liver transplantation, providing a potential target for new therapeutic interventions. We investigated the feasibility of in vivo bioluminescence imaging (BLI) to noninvasively quantify the spatiotemporal expression of HO-1 after warm hepatic ischemia in living animals. Luciferase activity was measured by BLI as an index of HO-1 transcription in transgenic reporter mice (Ho1-luc) at standardized time points after 60 minutes of warm hepatic ischemia. HO-1 mRNA levels were measured in postischemic livers of mice sacrificed at the same time points in separate experiments. Bioluminescent signals from postischemic liver lobes were first detected at 3 hours after reperfusion. Peak levels were reached at 9 hours, after which bioluminescent activity declined and returned to baseline values at 48 hours after reperfusion. Upregulation of HO-1 as detected by in vivo BLI was preceded by increased HO-1 mRNA expression and confirmed by enhanced immunohistochemical staining of hepatocytes. In conclusion, this study shows that in vivo BLI allows a sensitive assessment of HO-1 expression after hepatic ischemia in living animals. The capability of whole-body temporal imaging of HO-1 expression provides a valuable tool in the development of novel strategies to modulate HO-1 expression in liver transplantation.

Original language	English
Pages (from-to)	1634-9
Number of pages	6
Journal	Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society
Volume	12
Issue number	11
DOIs	https://doi.org/10.1002/lt.20852
Publication status	Published - Nov 2006

Keywords

Animals
Feasibility Studies
Heme Oxygenase-1
Immunohistochemistry
Ischemia
Liver
Luciferases
Luminescent Measurements
Male
Mice
Mice, Transgenic
RNA, Messenger
Reperfusion Injury
Staining and Labeling
Time Factors
Tissue Distribution
Warm Ischemia
Journal Article
Research Support, Non-U.S. Gov't

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10.1002/lt.20852

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Su, H., van Dam, G. M., Buis, C. I., Visser, D. S., Hesselink, J. W., Schuurs, T. A., Leuvenink, H. G. D., Contag, C. H., & Porte, R. J. (2006). Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice. Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, 12(11), 1634-9. https://doi.org/10.1002/lt.20852

Su, Huawei ; van Dam, Gooitzen M ; Buis, Carlijn I et al. / Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice. In: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society. 2006 ; Vol. 12, No. 11. pp. 1634-9.

@article{16b9e67b2b0f4e2bb57a8955fc94eada,

title = "Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice",

abstract = "Upregulation of heme oxygenase-1 (HO-1) has been proposed as a critical mechanism protecting against cellular stress during liver transplantation, providing a potential target for new therapeutic interventions. We investigated the feasibility of in vivo bioluminescence imaging (BLI) to noninvasively quantify the spatiotemporal expression of HO-1 after warm hepatic ischemia in living animals. Luciferase activity was measured by BLI as an index of HO-1 transcription in transgenic reporter mice (Ho1-luc) at standardized time points after 60 minutes of warm hepatic ischemia. HO-1 mRNA levels were measured in postischemic livers of mice sacrificed at the same time points in separate experiments. Bioluminescent signals from postischemic liver lobes were first detected at 3 hours after reperfusion. Peak levels were reached at 9 hours, after which bioluminescent activity declined and returned to baseline values at 48 hours after reperfusion. Upregulation of HO-1 as detected by in vivo BLI was preceded by increased HO-1 mRNA expression and confirmed by enhanced immunohistochemical staining of hepatocytes. In conclusion, this study shows that in vivo BLI allows a sensitive assessment of HO-1 expression after hepatic ischemia in living animals. The capability of whole-body temporal imaging of HO-1 expression provides a valuable tool in the development of novel strategies to modulate HO-1 expression in liver transplantation.",

keywords = "Animals, Feasibility Studies, Heme Oxygenase-1, Immunohistochemistry, Ischemia, Liver, Luciferases, Luminescent Measurements, Male, Mice, Mice, Transgenic, RNA, Messenger, Reperfusion Injury, Staining and Labeling, Time Factors, Tissue Distribution, Warm Ischemia, Journal Article, Research Support, Non-U.S. Gov't",

author = "Huawei Su and {van Dam}, {Gooitzen M} and Buis, {Carlijn I} and Visser, {Dorien S} and Hesselink, {Jan Willem} and Schuurs, {Theo A} and Leuvenink, {Henri G D} and Contag, {Christopher H.} and Porte, {Robert J}",

year = "2006",

month = nov,

doi = "10.1002/lt.20852",

language = "English",

volume = "12",

pages = "1634--9",

journal = "Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society",

issn = "1527-6465",

publisher = "Lippincott Williams and Wilkins",

number = "11",

}

Su, H, van Dam, GM, Buis, CI, Visser, DS, Hesselink, JW, Schuurs, TA, Leuvenink, HGD, Contag, CH & Porte, RJ 2006, 'Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice', Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, vol. 12, no. 11, pp. 1634-9. https://doi.org/10.1002/lt.20852

Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice. / Su, Huawei; van Dam, Gooitzen M; Buis, Carlijn I et al.
In: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Vol. 12, No. 11, 11.2006, p. 1634-9.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice

AU - Su, Huawei

AU - van Dam, Gooitzen M

AU - Buis, Carlijn I

AU - Visser, Dorien S

AU - Hesselink, Jan Willem

AU - Schuurs, Theo A

AU - Leuvenink, Henri G D

AU - Contag, Christopher H.

AU - Porte, Robert J

PY - 2006/11

Y1 - 2006/11

N2 - Upregulation of heme oxygenase-1 (HO-1) has been proposed as a critical mechanism protecting against cellular stress during liver transplantation, providing a potential target for new therapeutic interventions. We investigated the feasibility of in vivo bioluminescence imaging (BLI) to noninvasively quantify the spatiotemporal expression of HO-1 after warm hepatic ischemia in living animals. Luciferase activity was measured by BLI as an index of HO-1 transcription in transgenic reporter mice (Ho1-luc) at standardized time points after 60 minutes of warm hepatic ischemia. HO-1 mRNA levels were measured in postischemic livers of mice sacrificed at the same time points in separate experiments. Bioluminescent signals from postischemic liver lobes were first detected at 3 hours after reperfusion. Peak levels were reached at 9 hours, after which bioluminescent activity declined and returned to baseline values at 48 hours after reperfusion. Upregulation of HO-1 as detected by in vivo BLI was preceded by increased HO-1 mRNA expression and confirmed by enhanced immunohistochemical staining of hepatocytes. In conclusion, this study shows that in vivo BLI allows a sensitive assessment of HO-1 expression after hepatic ischemia in living animals. The capability of whole-body temporal imaging of HO-1 expression provides a valuable tool in the development of novel strategies to modulate HO-1 expression in liver transplantation.

AB - Upregulation of heme oxygenase-1 (HO-1) has been proposed as a critical mechanism protecting against cellular stress during liver transplantation, providing a potential target for new therapeutic interventions. We investigated the feasibility of in vivo bioluminescence imaging (BLI) to noninvasively quantify the spatiotemporal expression of HO-1 after warm hepatic ischemia in living animals. Luciferase activity was measured by BLI as an index of HO-1 transcription in transgenic reporter mice (Ho1-luc) at standardized time points after 60 minutes of warm hepatic ischemia. HO-1 mRNA levels were measured in postischemic livers of mice sacrificed at the same time points in separate experiments. Bioluminescent signals from postischemic liver lobes were first detected at 3 hours after reperfusion. Peak levels were reached at 9 hours, after which bioluminescent activity declined and returned to baseline values at 48 hours after reperfusion. Upregulation of HO-1 as detected by in vivo BLI was preceded by increased HO-1 mRNA expression and confirmed by enhanced immunohistochemical staining of hepatocytes. In conclusion, this study shows that in vivo BLI allows a sensitive assessment of HO-1 expression after hepatic ischemia in living animals. The capability of whole-body temporal imaging of HO-1 expression provides a valuable tool in the development of novel strategies to modulate HO-1 expression in liver transplantation.

KW - Animals

KW - Feasibility Studies

KW - Heme Oxygenase-1

KW - Immunohistochemistry

KW - Ischemia

KW - Liver

KW - Luciferases

KW - Luminescent Measurements

KW - Male

KW - Mice

KW - Mice, Transgenic

KW - RNA, Messenger

KW - Reperfusion Injury

KW - Staining and Labeling

KW - Time Factors

KW - Tissue Distribution

KW - Warm Ischemia

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/lt.20852

DO - 10.1002/lt.20852

M3 - Article

C2 - 17058249

SN - 1527-6465

VL - 12

SP - 1634

EP - 1639

JO - Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

JF - Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society

IS - 11

ER -

Su H, van Dam GM, Buis CI, Visser DS, Hesselink JW, Schuurs TA et al. Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice. Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society. 2006 Nov;12(11):1634-9. doi: 10.1002/lt.20852

Spatiotemporal expression of heme oxygenase-1 detected by in vivo bioluminescence after hepatic ischemia in HO-1/Luc mice

Abstract

Keywords

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