Spatial and temporal accumulation of mRNAs encoding two common lignin peroxidases in Phanerochaete chrysosporium

S.M. Moukha, H.A.B. Wosten, E.-J. Mylius, M. Asther, J.G.H. Wessels

Research output: Contribution to journalSpecial issueAcademicpeer-review

Abstract

Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8genes started simultaneously in the central area of 3- day-old colonies. With time the signals for both transcripts spread to more- peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8mRNA.
Original languageEnglish
Pages (from-to)3672-3678
Number of pages7
JournalJournal of Bacteriology
Volume175
Issue number11
Publication statusPublished - 5 Feb 1993

Keywords

  • lignin peroxidase
  • messenger RNA
  • enzyme localization
  • fungus
  • gene expression
  • in situ hybridization
  • nonhuman
  • Northern blotting
  • note
  • priority journal
  • transcription regulation

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