Spatial and Temporal Accumulation of Messenger-rnas Encoding 2 Common Lignin Peroxidases in Phanerochaete-chrysosporium

SM MOUKHA; H.A.B. Wösten; EJ MYLIUS; M ASTHER; JGH WESSELS

doi:10.1128/jb.175.11.3672-3678.1993

Spatial and Temporal Accumulation of Messenger-rnas Encoding 2 Common Lignin Peroxidases in Phanerochaete-chrysosporium

SM MOUKHA, H.A.B. Wösten, EJ MYLIUS, M ASTHER, JGH WESSELS

University of Groningen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both transcripts spread to more-peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA.

Original language	English
Pages (from-to)	3672-3678
Number of pages	7
Journal	Journal of Bacteriology
Volume	175
Issue number	11
DOIs	https://doi.org/10.1128/jb.175.11.3672-3678.1993
Publication status	Published - Jun 1993
Externally published	Yes

Keywords

White rot fungi
Degrading basidiomycete
Manganese peroxidase
Schizophyllum-commune
Wood
Localization
Degradation
Expression
Secretion
Proteins

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10.1128/jb.175.11.3672-3678.1993

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title = "Spatial and Temporal Accumulation of Messenger-rnas Encoding 2 Common Lignin Peroxidases in Phanerochaete-chrysosporium",

abstract = "Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both transcripts spread to more-peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA.",

keywords = "White rot fungi, Degrading basidiomycete, Manganese peroxidase, Schizophyllum-commune, Wood, Localization, Degradation, Expression, Secretion, Proteins",

author = "SM MOUKHA and H.A.B. W{\"o}sten and EJ MYLIUS and M ASTHER and JGH WESSELS",

year = "1993",

month = jun,

doi = "10.1128/jb.175.11.3672-3678.1993",

language = "English",

volume = "175",

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journal = "Journal of Bacteriology",

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T1 - Spatial and Temporal Accumulation of Messenger-rnas Encoding 2 Common Lignin Peroxidases in Phanerochaete-chrysosporium

AU - MOUKHA, SM

AU - Wösten, H.A.B.

AU - MYLIUS, EJ

AU - ASTHER, M

AU - WESSELS, JGH

PY - 1993/6

Y1 - 1993/6

N2 - Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both transcripts spread to more-peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA.

AB - Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both transcripts spread to more-peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA.

KW - White rot fungi

KW - Degrading basidiomycete

KW - Manganese peroxidase

KW - Schizophyllum-commune

KW - Wood

KW - Localization

KW - Degradation

KW - Expression

KW - Secretion

KW - Proteins

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DO - 10.1128/jb.175.11.3672-3678.1993

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SN - 0021-9193

VL - 175

SP - 3672

EP - 3678

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 11

ER -

Spatial and Temporal Accumulation of Messenger-rnas Encoding 2 Common Lignin Peroxidases in Phanerochaete-chrysosporium

Abstract

Keywords

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