Sorting of Streptomyces cell pellets using a complex object parametric analyzer and sorter

Marloes L C Petrus, G Jerre van Veluw, Han A B Wösten, Dennis Claessen, Han Wosten

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Streptomycetes are filamentous soil bacteria that are used in industry for the production of enzymes and antibiotics. When grown in bioreactors, these organisms form networks of interconnected hyphae, known as pellets, which are heterogeneous in size. Here we describe a method to analyze and sort mycelial pellets using a Complex Object Parametric Analyzer and Sorter (COPAS). Detailed instructions are given for the use of the instrument and the basic statistical analysis of the data. We furthermore describe how pellets can be sorted according to user-defined settings, which enables downstream processing such as the analysis of the RNA or protein content. Using this methodology the mechanism underlying heterogeneous growth can be tackled. This will be instrumental for improving streptomycetes as a cell factory, considering the fact that productivity correlates with pellet size.

Original languageEnglish
Article numbere51178
Number of pages6
JournalJ Biomed
Volume84
DOIs
Publication statusPublished - 2014

Keywords

  • Flow Cytometry
  • High-Throughput Screening Assays
  • Streptomyces coelicolor

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