TY - JOUR
T1 - Solid lipid nanoparticles for delivery of Calendula officinalis extract
AU - Arana, Lide
AU - Salado, Clarisa
AU - Vega, Sandra
AU - Aizpurua-Olaizola, Oier
AU - Arada, Igor de la
AU - Suarez, Tatiana
AU - Usobiaga, Aresatz
AU - Arrondo, José Luis R
AU - Alonso, Alicia
AU - Goñi, Félix M.
AU - Alkorta, Itziar
PY - 2015/11/1
Y1 - 2015/11/1
N2 - Solid lipid nanoparticles (SLN) composed of long-chain fatty acids (palmitic acid, stearic acid or arachidic acid), Epikuron 200 (purified phosphatidylcholine), and bile salts (cholate, taurocholate or taurodeoxycholate) have been prepared by dilution of a microemulsion. A total of five different systems were prepared, and characterized by photon correlation spectroscopy, transmission electron microscopy, differential scanning calorimetry, and infrared spectroscopy. The SLN formulation showing optimal properties (lowest size and polydispersity index and highest zeta potential) was obtained with stearic acid and taurodeoxycholate as cosurfactant. This formulation was loaded with Calendula officinalis extract, a natural compound used on ophthalmic formulations given its anti-inflammatory, emollient, and wound repairing activity. Calendula-loaded SLN preparations were characterized in order to determine loading capacity and entrapment efficiency. In vitro cytotoxicity and wound healing efficacy of Calendula-loaded SLN compared to that of a free plant extract were evaluated on a conjunctival epithelium cell line WKD. Our results suggest that this SLN formulation is a safe and solvent-free Calendula extract delivery system which could provide a controlled therapeutic alternative for reducing disease-related symptoms and improving epithelium repair in ocular surface.
AB - Solid lipid nanoparticles (SLN) composed of long-chain fatty acids (palmitic acid, stearic acid or arachidic acid), Epikuron 200 (purified phosphatidylcholine), and bile salts (cholate, taurocholate or taurodeoxycholate) have been prepared by dilution of a microemulsion. A total of five different systems were prepared, and characterized by photon correlation spectroscopy, transmission electron microscopy, differential scanning calorimetry, and infrared spectroscopy. The SLN formulation showing optimal properties (lowest size and polydispersity index and highest zeta potential) was obtained with stearic acid and taurodeoxycholate as cosurfactant. This formulation was loaded with Calendula officinalis extract, a natural compound used on ophthalmic formulations given its anti-inflammatory, emollient, and wound repairing activity. Calendula-loaded SLN preparations were characterized in order to determine loading capacity and entrapment efficiency. In vitro cytotoxicity and wound healing efficacy of Calendula-loaded SLN compared to that of a free plant extract were evaluated on a conjunctival epithelium cell line WKD. Our results suggest that this SLN formulation is a safe and solvent-free Calendula extract delivery system which could provide a controlled therapeutic alternative for reducing disease-related symptoms and improving epithelium repair in ocular surface.
KW - Calendula officinalis
KW - Cytotoxicity
KW - Differential scanning calorimetry
KW - Infrared spectroscopy
KW - Re-epithelialization
KW - Solid lipid nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84938073376&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfb.2015.07.020
DO - 10.1016/j.colsurfb.2015.07.020
M3 - Article
C2 - 26231862
AN - SCOPUS:84938073376
SN - 0927-7765
VL - 135
SP - 18
EP - 26
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
ER -