Soil-borne legacies of disease in Arabidopsis thaliana

Gilles Vismans; Jelle Spooren; Corné Pieterse; Peter Bakker; Roeland Berendsen

doi:10.1007/978-1-0716-1040-4_17

Soil-borne legacies of disease in Arabidopsis thaliana

Gilles Vismans, Jelle Spooren, Corné Pieterse, Peter Bakker, Roeland Berendsen

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

The rhizosphere microbiome of plants is essential for plant growth and health. Recent studies have shown that upon infection of leaves with a foliar pathogen, the composition of the root microbiome is altered and enriched with bacteria that in turn can systemically protect the plant against the foliar pathogen. This protective effect is extended to successive populations of plants that are grown on soil that was first conditioned by pathogen-infected plants, a phenomenon that was coined “the soil-borne legacy.” Here we provide a detailed protocol for soil-borne legacy experiments with the model plant Arabidopsis thaliana after infection with the obligate biotrophic pathogen Hyaloperonospora arabidopsidis. This protocol can easily be extended to infection with other pathogens or even infestation with herbivorous insects and can function as a blueprint for soil-borne legacy experiments with crop species.

Original language	English
Title of host publication	The Plant Microbiome
Subtitle of host publication	Methods and Protocols
Editors	Lilia C. Carvalhais, Paul G. Dennis
Publisher	Humana Press
Chapter	17
Pages	209-218
Number of pages	10
Edition	1
ISBN (Electronic)	978-1-0716-1040-4
ISBN (Print)	978-1-0716-1039-8, 978-1-0716-1042-8
DOIs	https://doi.org/10.1007/978-1-0716-1040-4_17
Publication status	Published - Jan 2021

Publication series

Name	Methods in Molecular Biology
Volume	2232
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
The authors would like to thank Annemiek Andel, Joyce Elberse and other members of the team of Guido Van den Ackerveken for maintenance of Hpa cultures. This work is supported the Dutch Technology Foundation TTW Perspective programme Back2Roots (Grant 14219).

Publisher Copyright:
© 2021, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Biotrophic pathogen
Disease resistance
Hyaloperonospora arabidopsidis (Hpa)
Plant-microbiome interactions
Rhizosphere

Access to Document

10.1007/978-1-0716-1040-4_17

MethodsMolBiol_Vismans_2021Final published version, 451 KB

Cite this

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Soil-borne legacies of disease in Arabidopsis thaliana. / Vismans, Gilles; Spooren, Jelle; Pieterse, Corné et al.
The Plant Microbiome: Methods and Protocols. ed. / Lilia C. Carvalhais; Paul G. Dennis. 1. ed. Humana Press, 2021. p. 209-218 (Methods in Molecular Biology; Vol. 2232).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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Soil-borne legacies of disease in Arabidopsis thaliana

Abstract

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Bibliographical note

Keywords

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