Skin tissue sample collection, sample homogenization, and analyte extraction strategies for liquid chromatographic mass spectrometry quantification of pharmaceutical compounds

Ignace C Roseboom, Hilde Rosing, Jos H Beijnen, Thomas P C Dorlo

Research output: Contribution to journalReview articlepeer-review

Abstract

Quantification of pharmaceutical compounds in skin tissue is challenging because of low expected concentrations, small typical sample volumes, and the hard nature of the skin structure itself. This review provides a comprehensive overview of sample collection, sample homogenization and analyte extraction methods that have been used to quantify pharmaceutical compounds in skin tissue, obtained from animals and humans, using liquid chromatography-mass spectrometry. For each step in the process of sample collection to sample extraction, methods are compared to discuss challenges and provide practical guidance. Furthermore, liquid chromatographic-mass spectrometry considerations regarding the quality and complexity of skin tissue sample measurements are discussed, with emphasis on analyte recovery and matrix effects. Given that the true recovery of analytes from skin tissue is difficult to assess, the extent of homogenization plays a crucial role in the accuracy of quantification. Chemical or enzymatic solubilization of skin tissue samples would therefore be preferable as homogenization method.

Original languageEnglish
Article number113590
Number of pages9
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume191
Issue number113590
DOIs
Publication statusPublished - 30 Nov 2020

Keywords

  • liquid chromatography tandem mass
  • spectrometry
  • dermal
  • tissue homogenization
  • Sample preparation
  • bioanalysis
  • quantification

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