Single molecule quantitation and sequencing of rare translocations using microfluidic nested digital PCR

J. Shuga; Y. Zeng; R. Novak; Q. Lan; X. Tang; N. Rothman; R. Vermeulen; L. Li; A. Hubbard; L. Zhang; R.A. Mathies; M.T. Smith

doi:10.1093/nar/gkt613

Single molecule quantitation and sequencing of rare translocations using microfluidic nested digital PCR

J. Shuga, Y. Zeng, R. Novak, Q. Lan, X. Tang, N. Rothman, R. Vermeulen, L. Li, A. Hubbard, L. Zhang, R.A. Mathies, M.T. Smith

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Cancers are heterogeneous and genetically unstable. New methods are needed that provide the sensitivity and specificity to query single cells at the genetic loci that drive cancer progression, thereby enabling researchers to study the progression of individual tumors. Here, we report the development and application of a bead-based hemi-nested microfluidic droplet digital PCR (dPCR) technology to achieve 'quantitative' measurement and single-molecule sequencing of somatically acquired carcinogenic translocations at extremely low levels (

Original language	English
Pages (from-to)	e159
Number of pages	1
Journal	Nucleic Acids Research
Volume	41
Issue number	16
DOIs	https://doi.org/10.1093/nar/gkt613
Publication status	Published - 2013

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abstract = "Cancers are heterogeneous and genetically unstable. New methods are needed that provide the sensitivity and specificity to query single cells at the genetic loci that drive cancer progression, thereby enabling researchers to study the progression of individual tumors. Here, we report the development and application of a bead-based hemi-nested microfluidic droplet digital PCR (dPCR) technology to achieve 'quantitative' measurement and single-molecule sequencing of somatically acquired carcinogenic translocations at extremely low levels (",

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AU - Tang, X.

AU - Rothman, N.

AU - Vermeulen, R.

AU - Li, L.

AU - Hubbard, A.

AU - Zhang, L.

AU - Mathies, R.A.

AU - Smith, M.T.

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AB - Cancers are heterogeneous and genetically unstable. New methods are needed that provide the sensitivity and specificity to query single cells at the genetic loci that drive cancer progression, thereby enabling researchers to study the progression of individual tumors. Here, we report the development and application of a bead-based hemi-nested microfluidic droplet digital PCR (dPCR) technology to achieve 'quantitative' measurement and single-molecule sequencing of somatically acquired carcinogenic translocations at extremely low levels (

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JO - Nucleic Acids Research

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Single molecule quantitation and sequencing of rare translocations using microfluidic nested digital PCR

Abstract

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