@inproceedings{33e97ac7b1954f0a9376071493c80fda,
title = "Single molecule, CdSe/ZnS quantum dot and gold nanoparticle detection in live neurons",
abstract = "We applied single-molecule fluorescence microscopy (using organic dyes or semiconductor quantum dots) to study the lateral diffusion of glutamate receptors (AMPA and NMDA) in live synapses. We directly imaged glutamate receptors movements inside and outside synapses of live cultured hippocampal neurons. We could record exchanges of receptors through lateral diffusion between these different membrane compartments. In addition, our data suggest that this lateral diffusion might be regulated by neuronal activity. To overcome the photobleaching problem inherent to fluorescence techniques we recently developed new optical methods for the detection of individual metallic nanoparticles. We can now detect signatures of diffusing AMPA receptors labeled with 10 nm gold nanoparticles on live neurons.",
keywords = "Fluorescence microscopy, Glutamate receptors, Gold nanoparticles, Neuron, Quantum dots, Single molecules",
author = "D. Lasne and L. Cognet and S. Berciaud and Blab, {G. A.} and L. Groc and M. Heine and D. Choquet and B. Lounis",
year = "2006",
doi = "10.1117/12.647788",
language = "English",
isbn = "0819461385",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "SPIE",
booktitle = "Colloidal Quantum Dots for Biomedical Applications",
address = "United States",
note = "Colloidal Quantum Dots for Biomedical Applications ; Conference date: 22-01-2006 Through 24-01-2006",
}