Simultaneous determination of flucytosine and fluorouracil in human plasma by high-performance liquid chromatography

Javier Sastre Torano, A Vermes, HJ Guchelaar*

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    A validated, sensitive and precise reversed-phase high-performance liquid chromatographic method for the simultaneous determination of 5-flucytosine (5-FC) and 5-fluorouracil (5-FU) in human plasma is described. Two compounds, 5-methylcytosine (5-MG) and 5-chlorouracil (5-CU), were used as internal standards for the determination of 5-FC and 5-FU, respectively. Plasma samples were deproteinized with trichloroacetic acid and chromatographed on an octylsilica column, maintained at 30 degreesC during elution, using a 0.04 M phosphate buffer, pH 7.0, as eleunt. Spectrophotometric diode array detection was used at 266 nm. 5-FC, 5-FU, 5-MG and 5-CU were found to have retention times of 4.8, 5.8, 7.7 and 11.0 min respectively. Recoveries of 91-120% with reproducibility and repeatability coefficients of variation of 0.8-6% were obtained. Mean correlation coefficients of 0.99989 and 0.9995 were found for the linear calibration curves (n = 2) of 5-FC (4.816-192.6 mg/l) and 5-FU (0.05368-5.368 mg/l), respectively. The limits of quantitation were 0.3 mg/l for 5-FC and 0.05 mg/l for 5-FU. Copyright (C) 2001 John Wiley & Sons, Ltd.

    Original languageEnglish
    Pages (from-to)89-94
    Number of pages6
    JournalBiomedical Chromatography
    Volume15
    Issue number2
    Publication statusPublished - Apr 2001

    Keywords

    • ACTIVE METABOLITES
    • 5-FLUOROURACIL
    • TISSUE
    • SERUM
    • TOXICITY
    • ASSAY
    • 5-FLUOROCYTOSINE
    • HPLC

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