Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC-MS/MS

Milly E. Attema-de Jonge; Judith M. Bekkers; Heleen M. Oudemans-van Straaten; Rolf W. Sparidans; Eric J F Franssen

doi:10.1016/j.jchromb.2007.12.008

Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC-MS/MS

Milly E. Attema-de Jonge^*
, Judith M. Bekkers
, Heleen M. Oudemans-van Straaten
, Rolf W. Sparidans
, Eric J F Franssen

^*Corresponding author for this work

Pharmacoepidemiology and Clinical Pharmacology

Onze Lieve Vrouwe Gasthuis
University Medical Center Utrecht

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

After oral administration of tobramycin, as part of selective decontamination of the digestive tract (SDD) in critically ill patients, absorption of tobramycin from the gut into the blood may take place. To quantify low concentrations of tobramycin in human plasma, we developed and validated a simple (sample pre-treatment consisting of protein precipitation with acetonitrile using 200 μl plasma), rapid (runtime 3 min using a Pathfinder MR reversed-phase column) and sensitive (concentration range of 0.05-1.0 mg/l using MS/MS detection) method.

Original language	English
Pages (from-to)	257-262
Number of pages	6
Journal	Journal of chromatography. B
Volume	862
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jchromb.2007.12.008
Publication status	Published - 1 Feb 2008

Keywords

HPLC-MS/MS
Selective decontamination of the digestive tract
Sisomicin
Tobramycin

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10.1016/j.jchromb.2007.12.008

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title = "Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC-MS/MS",

abstract = "After oral administration of tobramycin, as part of selective decontamination of the digestive tract (SDD) in critically ill patients, absorption of tobramycin from the gut into the blood may take place. To quantify low concentrations of tobramycin in human plasma, we developed and validated a simple (sample pre-treatment consisting of protein precipitation with acetonitrile using 200 μl plasma), rapid (runtime 3 min using a Pathfinder MR reversed-phase column) and sensitive (concentration range of 0.05-1.0 mg/l using MS/MS detection) method.",

keywords = "HPLC-MS/MS, Selective decontamination of the digestive tract, Sisomicin, Tobramycin",

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T1 - Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC-MS/MS

AU - Attema-de Jonge, Milly E.

AU - Bekkers, Judith M.

AU - Oudemans-van Straaten, Heleen M.

AU - Sparidans, Rolf W.

AU - Franssen, Eric J F

PY - 2008/2/1

Y1 - 2008/2/1

N2 - After oral administration of tobramycin, as part of selective decontamination of the digestive tract (SDD) in critically ill patients, absorption of tobramycin from the gut into the blood may take place. To quantify low concentrations of tobramycin in human plasma, we developed and validated a simple (sample pre-treatment consisting of protein precipitation with acetonitrile using 200 μl plasma), rapid (runtime 3 min using a Pathfinder MR reversed-phase column) and sensitive (concentration range of 0.05-1.0 mg/l using MS/MS detection) method.

AB - After oral administration of tobramycin, as part of selective decontamination of the digestive tract (SDD) in critically ill patients, absorption of tobramycin from the gut into the blood may take place. To quantify low concentrations of tobramycin in human plasma, we developed and validated a simple (sample pre-treatment consisting of protein precipitation with acetonitrile using 200 μl plasma), rapid (runtime 3 min using a Pathfinder MR reversed-phase column) and sensitive (concentration range of 0.05-1.0 mg/l using MS/MS detection) method.

KW - HPLC-MS/MS

KW - Selective decontamination of the digestive tract

KW - Sisomicin

KW - Tobramycin

UR - https://www.scopus.com/pages/publications/38749142900

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M3 - Article

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AN - SCOPUS:38749142900

SN - 1570-0232

VL - 862

SP - 257

EP - 262

JO - Journal of chromatography. B

JF - Journal of chromatography. B

IS - 1-2

ER -

Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC-MS/MS

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Keywords

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