TY - JOUR
T1 - Simple and sensitive method for quantification of low tobramycin concentrations in human plasma using HPLC-MS/MS
AU - Attema-de Jonge, Milly E.
AU - Bekkers, Judith M.
AU - Oudemans-van Straaten, Heleen M.
AU - Sparidans, Rolf W.
AU - Franssen, Eric J F
PY - 2008/2/1
Y1 - 2008/2/1
N2 - After oral administration of tobramycin, as part of selective decontamination of the digestive tract (SDD) in critically ill patients, absorption of tobramycin from the gut into the blood may take place. To quantify low concentrations of tobramycin in human plasma, we developed and validated a simple (sample pre-treatment consisting of protein precipitation with acetonitrile using 200 μl plasma), rapid (runtime 3 min using a Pathfinder MR reversed-phase column) and sensitive (concentration range of 0.05-1.0 mg/l using MS/MS detection) method.
AB - After oral administration of tobramycin, as part of selective decontamination of the digestive tract (SDD) in critically ill patients, absorption of tobramycin from the gut into the blood may take place. To quantify low concentrations of tobramycin in human plasma, we developed and validated a simple (sample pre-treatment consisting of protein precipitation with acetonitrile using 200 μl plasma), rapid (runtime 3 min using a Pathfinder MR reversed-phase column) and sensitive (concentration range of 0.05-1.0 mg/l using MS/MS detection) method.
KW - HPLC-MS/MS
KW - Selective decontamination of the digestive tract
KW - Sisomicin
KW - Tobramycin
UR - http://www.scopus.com/inward/record.url?scp=38749142900&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2007.12.008
DO - 10.1016/j.jchromb.2007.12.008
M3 - Article
C2 - 18178137
AN - SCOPUS:38749142900
SN - 1570-0232
VL - 862
SP - 257
EP - 262
JO - Journal of chromatography. B
JF - Journal of chromatography. B
IS - 1-2
ER -