Shifted T-cell polarisation after agricultural dust exposure in mice and men

P Robbe; E A J Spierenburg; C Draijer; C A Brandsma; E Telenga; A J M van Oosterhout; M van den Berge; M Luinge; B N Melgert; D Heederik; W Timens; I M Wouters; M N Hylkema

doi:10.1136/thoraxjnl-2013-204295

Shifted T-cell polarisation after agricultural dust exposure in mice and men

P Robbe, E A J Spierenburg, C Draijer, C A Brandsma, E Telenga, A J M van Oosterhout, M van den Berge, M Luinge, B N Melgert, D Heederik, W Timens, I M Wouters, M N Hylkema

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

RATIONALE: A low prevalence of asthma and atopy has been shown in farmers and agricultural workers. However, in these workers, a higher prevalence of respiratory symptoms has been reported, in which T helper 1 (Th1) and/or Th17 responses may play a role.

AIM: We investigated the effect of exposure to dust extracts (DEs) from different farms on airway inflammation and T-cell polarisation in a mouse model and assessed T-cell polarisation in agricultural workers from the same farms.

METHODS: DEs were prepared from settled dust collected at cattle and pig farms and bulb and onion industries. Mice were exposed to phosphate-buffered saline (PBS), DEs, house dust mite (HDM) or HDM+DE via nasal instillation, four times per week during 5 weeks. Hyperresponsiveness, airway inflammation, IgE levels and T-cell polarisation were assessed. Th-cell and T cytotoxic (Tc)-cell subsets were investigated in peripheral blood samples from 33 agricultural workers and 9 non-exposed controls.

RESULTS: DEs induced interleukin(IL)-17, IL-1β and IL-6 in mouse lung homogenates. DE-exposed mice had more mixed inflammatory infiltrates in the lungs, and more neutrophils compared with PBS-exposed mice. DEs protected against the HDM-induced Th2 response and methacholine hyperresponsiveness. Interestingly, occupationally exposed humans had higher frequencies of Th cells spontaneously expressing IL-17 and interferon γ compared with controls.

CONCLUSION: Chronic exposure to different types of farm dust induces a Th/Tc-17 inflammatory response in mice and agricultural workers. This may contribute to the low prevalence of Th2-related diseases but may constitute a risk for other chronic respiratory diseases.

Original language	English
Pages (from-to)	630-637
Number of pages	8
Journal	Thorax
Volume	69
Issue number	7
DOIs	https://doi.org/10.1136/thoraxjnl-2013-204295
Publication status	Published - Jul 2014

Bibliographical note

Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

Keywords

Agriculture
Animals
Bronchial Provocation Tests
Disease Models, Animal
Dust
Environmental Exposure
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Humans
Immunoenzyme Techniques
Immunoglobulin E
Inflammation
Interleukin-17
Interleukin-1beta
Interleukin-6
Lung
Mice
Mice, Inbred BALB C
Pyroglyphidae
T-Lymphocytes
Th1 Cells
Th2 Cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1136/thoraxjnl-2013-204295

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title = "Shifted T-cell polarisation after agricultural dust exposure in mice and men",

abstract = "RATIONALE: A low prevalence of asthma and atopy has been shown in farmers and agricultural workers. However, in these workers, a higher prevalence of respiratory symptoms has been reported, in which T helper 1 (Th1) and/or Th17 responses may play a role.AIM: We investigated the effect of exposure to dust extracts (DEs) from different farms on airway inflammation and T-cell polarisation in a mouse model and assessed T-cell polarisation in agricultural workers from the same farms.METHODS: DEs were prepared from settled dust collected at cattle and pig farms and bulb and onion industries. Mice were exposed to phosphate-buffered saline (PBS), DEs, house dust mite (HDM) or HDM+DE via nasal instillation, four times per week during 5 weeks. Hyperresponsiveness, airway inflammation, IgE levels and T-cell polarisation were assessed. Th-cell and T cytotoxic (Tc)-cell subsets were investigated in peripheral blood samples from 33 agricultural workers and 9 non-exposed controls.RESULTS: DEs induced interleukin(IL)-17, IL-1β and IL-6 in mouse lung homogenates. DE-exposed mice had more mixed inflammatory infiltrates in the lungs, and more neutrophils compared with PBS-exposed mice. DEs protected against the HDM-induced Th2 response and methacholine hyperresponsiveness. Interestingly, occupationally exposed humans had higher frequencies of Th cells spontaneously expressing IL-17 and interferon γ compared with controls.CONCLUSION: Chronic exposure to different types of farm dust induces a Th/Tc-17 inflammatory response in mice and agricultural workers. This may contribute to the low prevalence of Th2-related diseases but may constitute a risk for other chronic respiratory diseases.",

keywords = "Agriculture, Animals, Bronchial Provocation Tests, Disease Models, Animal, Dust, Environmental Exposure, Enzyme-Linked Immunosorbent Assay, Female, Flow Cytometry, Humans, Immunoenzyme Techniques, Immunoglobulin E, Inflammation, Interleukin-17, Interleukin-1beta, Interleukin-6, Lung, Mice, Mice, Inbred BALB C, Pyroglyphidae, T-Lymphocytes, Th1 Cells, Th2 Cells",

author = "P Robbe and Spierenburg, \{E A J\} and C Draijer and Brandsma, \{C A\} and E Telenga and \{van Oosterhout\}, \{A J M\} and \{van den Berge\}, M and M Luinge and Melgert, \{B N\} and D Heederik and W Timens and Wouters, \{I M\} and Hylkema, \{M N\}",

note = "Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.",

year = "2014",

month = jul,

doi = "10.1136/thoraxjnl-2013-204295",

language = "English",

volume = "69",

pages = "630--637",

journal = "Thorax",

issn = "0040-6376",

publisher = "BMJ Publishing Group",

number = "7",

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TY - JOUR

T1 - Shifted T-cell polarisation after agricultural dust exposure in mice and men

AU - Robbe, P

AU - Spierenburg, E A J

AU - Draijer, C

AU - Brandsma, C A

AU - Telenga, E

AU - van Oosterhout, A J M

AU - van den Berge, M

AU - Luinge, M

AU - Melgert, B N

AU - Heederik, D

AU - Timens, W

AU - Wouters, I M

AU - Hylkema, M N

N1 - Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.

PY - 2014/7

Y1 - 2014/7

N2 - RATIONALE: A low prevalence of asthma and atopy has been shown in farmers and agricultural workers. However, in these workers, a higher prevalence of respiratory symptoms has been reported, in which T helper 1 (Th1) and/or Th17 responses may play a role.AIM: We investigated the effect of exposure to dust extracts (DEs) from different farms on airway inflammation and T-cell polarisation in a mouse model and assessed T-cell polarisation in agricultural workers from the same farms.METHODS: DEs were prepared from settled dust collected at cattle and pig farms and bulb and onion industries. Mice were exposed to phosphate-buffered saline (PBS), DEs, house dust mite (HDM) or HDM+DE via nasal instillation, four times per week during 5 weeks. Hyperresponsiveness, airway inflammation, IgE levels and T-cell polarisation were assessed. Th-cell and T cytotoxic (Tc)-cell subsets were investigated in peripheral blood samples from 33 agricultural workers and 9 non-exposed controls.RESULTS: DEs induced interleukin(IL)-17, IL-1β and IL-6 in mouse lung homogenates. DE-exposed mice had more mixed inflammatory infiltrates in the lungs, and more neutrophils compared with PBS-exposed mice. DEs protected against the HDM-induced Th2 response and methacholine hyperresponsiveness. Interestingly, occupationally exposed humans had higher frequencies of Th cells spontaneously expressing IL-17 and interferon γ compared with controls.CONCLUSION: Chronic exposure to different types of farm dust induces a Th/Tc-17 inflammatory response in mice and agricultural workers. This may contribute to the low prevalence of Th2-related diseases but may constitute a risk for other chronic respiratory diseases.

AB - RATIONALE: A low prevalence of asthma and atopy has been shown in farmers and agricultural workers. However, in these workers, a higher prevalence of respiratory symptoms has been reported, in which T helper 1 (Th1) and/or Th17 responses may play a role.AIM: We investigated the effect of exposure to dust extracts (DEs) from different farms on airway inflammation and T-cell polarisation in a mouse model and assessed T-cell polarisation in agricultural workers from the same farms.METHODS: DEs were prepared from settled dust collected at cattle and pig farms and bulb and onion industries. Mice were exposed to phosphate-buffered saline (PBS), DEs, house dust mite (HDM) or HDM+DE via nasal instillation, four times per week during 5 weeks. Hyperresponsiveness, airway inflammation, IgE levels and T-cell polarisation were assessed. Th-cell and T cytotoxic (Tc)-cell subsets were investigated in peripheral blood samples from 33 agricultural workers and 9 non-exposed controls.RESULTS: DEs induced interleukin(IL)-17, IL-1β and IL-6 in mouse lung homogenates. DE-exposed mice had more mixed inflammatory infiltrates in the lungs, and more neutrophils compared with PBS-exposed mice. DEs protected against the HDM-induced Th2 response and methacholine hyperresponsiveness. Interestingly, occupationally exposed humans had higher frequencies of Th cells spontaneously expressing IL-17 and interferon γ compared with controls.CONCLUSION: Chronic exposure to different types of farm dust induces a Th/Tc-17 inflammatory response in mice and agricultural workers. This may contribute to the low prevalence of Th2-related diseases but may constitute a risk for other chronic respiratory diseases.

KW - Agriculture

KW - Animals

KW - Bronchial Provocation Tests

KW - Disease Models, Animal

KW - Dust

KW - Environmental Exposure

KW - Enzyme-Linked Immunosorbent Assay

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Immunoenzyme Techniques

KW - Immunoglobulin E

KW - Inflammation

KW - Interleukin-17

KW - Interleukin-1beta

KW - Interleukin-6

KW - Lung

KW - Mice

KW - Mice, Inbred BALB C

KW - Pyroglyphidae

KW - T-Lymphocytes

KW - Th1 Cells

KW - Th2 Cells

U2 - 10.1136/thoraxjnl-2013-204295

DO - 10.1136/thoraxjnl-2013-204295

M3 - Article

C2 - 24536057

SN - 0040-6376

VL - 69

SP - 630

EP - 637

JO - Thorax

JF - Thorax

IS - 7

ER -

Shifted T-cell polarisation after agricultural dust exposure in mice and men

Abstract

Bibliographical note

Keywords

UN SDGs

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