Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus

Judith M A van den Brand; Koert J Stittelaar; Geert van Amerongen; Guus F Rimmelzwaan; James Simon; Emmie de Wit; Vincent J Munster; Theo M Bestebroer; Ron A M Fouchier; Thijs Kuiken; Albert D M E Osterhaus

doi:10.1086/651132

Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus

Judith M A van den Brand, Koert J Stittelaar, Geert van Amerongen, Guus F Rimmelzwaan, James Simon, Emmie de Wit, Vincent J Munster, Theo M Bestebroer, Ron A M Fouchier, Thijs Kuiken, Albert D M E Osterhaus

Department of Virology, Erasmus Medical Centre, Rotterdam, The Netherlands.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: The newly emerged influenza A(H1N1) virus (new H1N1 virus) is causing the first influenza pandemic of this century. Three influenza pandemics of the previous century caused variable mortality, which largely depended on the development of severe pneumonia. However, the ability of the new H1N1 virus to cause pneumonia is poorly understood.

METHODS: The new H1N1 virus was inoculated intratracheally into ferrets. Its ability to cause pneumonia was compared with that of seasonal influenza H1N1 virus and highly pathogenic avian influenza (HPAI) H5N1 virus by using clinical, virological, and pathological analyses.

RESULTS: Our results showed that the new H1N1 virus causes pneumonia in ferrets intermediate in severity between that caused by seasonal H1N1 virus and by HPAI H5N1 virus. The new H1N1 virus replicated well throughout the lower respiratory tract and more extensively than did both seasonal H1N1 virus (which replicated mainly in the bronchi) and HPAI H5N1 virus (which replicated mainly in the alveoli). High loads of new H1N1 virus in lung tissue were associated with diffuse alveolar damage and mortality.

CONCLUSIONS: The new H1N1 virus may be intrinsically more pathogenic for humans than is seasonal H1N1 virus.

Original language	English
Pages (from-to)	993-9
Number of pages	7
Journal	Journal of Infectious Diseases
Volume	201
Issue number	7
DOIs	https://doi.org/10.1086/651132
Publication status	Published - 1 Apr 2010

Keywords

Analysis of Variance
Animals
Body Temperature
Body Weight
Cell Line
Female
Ferrets
Immunohistochemistry
Influenza A Virus, H1N1 Subtype
Influenza A Virus, H5N1 Subtype
Lung
Male
Organ Size
Orthomyxoviridae Infections
Pneumonia, Viral
Severity of Illness Index
Viral Load
Journal Article
Research Support, Non-U.S. Gov't

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10.1086/651132

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van den Brand, J. M. A., Stittelaar, K. J., Amerongen, G. V., Rimmelzwaan, G. F., Simon, J., de Wit, E., Munster, V. J., Bestebroer, T. M., Fouchier, R. A. M., Kuiken, T., & Osterhaus, A. D. M. E. (2010). Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus. Journal of Infectious Diseases, 201(7), 993-9. https://doi.org/10.1086/651132

@article{b1a750ea5bad4e5bb50bc1469b1f0bac,

title = "Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus",

abstract = "BACKGROUND: The newly emerged influenza A(H1N1) virus (new H1N1 virus) is causing the first influenza pandemic of this century. Three influenza pandemics of the previous century caused variable mortality, which largely depended on the development of severe pneumonia. However, the ability of the new H1N1 virus to cause pneumonia is poorly understood.METHODS: The new H1N1 virus was inoculated intratracheally into ferrets. Its ability to cause pneumonia was compared with that of seasonal influenza H1N1 virus and highly pathogenic avian influenza (HPAI) H5N1 virus by using clinical, virological, and pathological analyses.RESULTS: Our results showed that the new H1N1 virus causes pneumonia in ferrets intermediate in severity between that caused by seasonal H1N1 virus and by HPAI H5N1 virus. The new H1N1 virus replicated well throughout the lower respiratory tract and more extensively than did both seasonal H1N1 virus (which replicated mainly in the bronchi) and HPAI H5N1 virus (which replicated mainly in the alveoli). High loads of new H1N1 virus in lung tissue were associated with diffuse alveolar damage and mortality.CONCLUSIONS: The new H1N1 virus may be intrinsically more pathogenic for humans than is seasonal H1N1 virus.",

keywords = "Analysis of Variance, Animals, Body Temperature, Body Weight, Cell Line, Female, Ferrets, Immunohistochemistry, Influenza A Virus, H1N1 Subtype, Influenza A Virus, H5N1 Subtype, Lung, Male, Organ Size, Orthomyxoviridae Infections, Pneumonia, Viral, Severity of Illness Index, Viral Load, Journal Article, Research Support, Non-U.S. Gov't",

author = "{van den Brand}, {Judith M A} and Stittelaar, {Koert J} and Amerongen, {Geert van} and Rimmelzwaan, {Guus F} and James Simon and {de Wit}, Emmie and Munster, {Vincent J} and Bestebroer, {Theo M} and Fouchier, {Ron A M} and Thijs Kuiken and Osterhaus, {Albert D M E}",

year = "2010",

month = apr,

day = "1",

doi = "10.1086/651132",

language = "English",

volume = "201",

pages = "993--9",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "7",

}

van den Brand, JMA, Stittelaar, KJ, Amerongen, GV, Rimmelzwaan, GF, Simon, J, de Wit, E, Munster, VJ, Bestebroer, TM, Fouchier, RAM, Kuiken, T & Osterhaus, ADME 2010, 'Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus', Journal of Infectious Diseases, vol. 201, no. 7, pp. 993-9. https://doi.org/10.1086/651132

Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus. / van den Brand, Judith M A; Stittelaar, Koert J; Amerongen, Geert van et al.
In: Journal of Infectious Diseases, Vol. 201, No. 7, 01.04.2010, p. 993-9.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus

AU - van den Brand, Judith M A

AU - Stittelaar, Koert J

AU - Amerongen, Geert van

AU - Rimmelzwaan, Guus F

AU - Simon, James

AU - de Wit, Emmie

AU - Munster, Vincent J

AU - Bestebroer, Theo M

AU - Fouchier, Ron A M

AU - Kuiken, Thijs

AU - Osterhaus, Albert D M E

PY - 2010/4/1

Y1 - 2010/4/1

N2 - BACKGROUND: The newly emerged influenza A(H1N1) virus (new H1N1 virus) is causing the first influenza pandemic of this century. Three influenza pandemics of the previous century caused variable mortality, which largely depended on the development of severe pneumonia. However, the ability of the new H1N1 virus to cause pneumonia is poorly understood.METHODS: The new H1N1 virus was inoculated intratracheally into ferrets. Its ability to cause pneumonia was compared with that of seasonal influenza H1N1 virus and highly pathogenic avian influenza (HPAI) H5N1 virus by using clinical, virological, and pathological analyses.RESULTS: Our results showed that the new H1N1 virus causes pneumonia in ferrets intermediate in severity between that caused by seasonal H1N1 virus and by HPAI H5N1 virus. The new H1N1 virus replicated well throughout the lower respiratory tract and more extensively than did both seasonal H1N1 virus (which replicated mainly in the bronchi) and HPAI H5N1 virus (which replicated mainly in the alveoli). High loads of new H1N1 virus in lung tissue were associated with diffuse alveolar damage and mortality.CONCLUSIONS: The new H1N1 virus may be intrinsically more pathogenic for humans than is seasonal H1N1 virus.

AB - BACKGROUND: The newly emerged influenza A(H1N1) virus (new H1N1 virus) is causing the first influenza pandemic of this century. Three influenza pandemics of the previous century caused variable mortality, which largely depended on the development of severe pneumonia. However, the ability of the new H1N1 virus to cause pneumonia is poorly understood.METHODS: The new H1N1 virus was inoculated intratracheally into ferrets. Its ability to cause pneumonia was compared with that of seasonal influenza H1N1 virus and highly pathogenic avian influenza (HPAI) H5N1 virus by using clinical, virological, and pathological analyses.RESULTS: Our results showed that the new H1N1 virus causes pneumonia in ferrets intermediate in severity between that caused by seasonal H1N1 virus and by HPAI H5N1 virus. The new H1N1 virus replicated well throughout the lower respiratory tract and more extensively than did both seasonal H1N1 virus (which replicated mainly in the bronchi) and HPAI H5N1 virus (which replicated mainly in the alveoli). High loads of new H1N1 virus in lung tissue were associated with diffuse alveolar damage and mortality.CONCLUSIONS: The new H1N1 virus may be intrinsically more pathogenic for humans than is seasonal H1N1 virus.

KW - Analysis of Variance

KW - Animals

KW - Body Temperature

KW - Body Weight

KW - Cell Line

KW - Female

KW - Ferrets

KW - Immunohistochemistry

KW - Influenza A Virus, H1N1 Subtype

KW - Influenza A Virus, H5N1 Subtype

KW - Lung

KW - Male

KW - Organ Size

KW - Orthomyxoviridae Infections

KW - Pneumonia, Viral

KW - Severity of Illness Index

KW - Viral Load

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1086/651132

DO - 10.1086/651132

M3 - Article

C2 - 20187747

SN - 0022-1899

VL - 201

SP - 993

EP - 999

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

IS - 7

ER -

Severity of pneumonia due to new H1N1 influenza virus in ferrets is intermediate between that due to seasonal H1N1 virus and highly pathogenic avian influenza H5N1 virus

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Keywords

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