Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies

Nisreen M A Okba; Ivy Widjaja; Wentao Li; Corine H GeurtsvanKessel; Elmoubasher A B A Farag; Mohammed Al-Hajri; Wan Beom Park; Myoung-Don Oh; Chantal B M E Reusken; Marion P G Koopmans; Berend-Jan Bosch; Bart L Haagmans

doi:10.3201/eid2605.190921

Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies

Nisreen M A Okba
, Ivy Widjaja
, Wentao Li
, Corine H GeurtsvanKessel
, Elmoubasher A B A Farag
, Mohammed Al-Hajri
, Wan Beom Park
, Myoung-Don Oh
, Chantal B M E Reusken
, Marion P G Koopmans
, Berend-Jan Bosch
, Bart L Haagmans

Virology

Research output: Contribution to journal › Letter › Academic › peer-review

Abstract

We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome.

Original language	English
Pages (from-to)	1024-1027
Journal	Emerging Infectious Diseases
Volume	26
Issue number	5
DOIs	https://doi.org/10.3201/eid2605.190921
Publication status	Published - 2020

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Okba, N. M. A., Widjaja, I., Li, W., GeurtsvanKessel, C. H., Farag, E. A. B. A., Al-Hajri, M., Park, W. B., Oh, M.-D., Reusken, C. B. M. E., Koopmans, M. P. G., Bosch, B.-J., & Haagmans, B. L. (2020). Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies. Emerging Infectious Diseases, 26(5), 1024-1027. https://doi.org/10.3201/eid2605.190921

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title = "Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies",

abstract = "We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome.",

author = "Okba, \{Nisreen M A\} and Ivy Widjaja and Wentao Li and GeurtsvanKessel, \{Corine H\} and Farag, \{Elmoubasher A B A\} and Mohammed Al-Hajri and Park, \{Wan Beom\} and Myoung-Don Oh and Reusken, \{Chantal B M E\} and Koopmans, \{Marion P G\} and Berend-Jan Bosch and Haagmans, \{Bart L\}",

year = "2020",

doi = "10.3201/eid2605.190921",

language = "English",

volume = "26",

pages = "1024--1027",

journal = "Emerging Infectious Diseases",

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T1 - Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies

AU - Okba, Nisreen M A

AU - Widjaja, Ivy

AU - Li, Wentao

AU - GeurtsvanKessel, Corine H

AU - Farag, Elmoubasher A B A

AU - Al-Hajri, Mohammed

AU - Park, Wan Beom

AU - Oh, Myoung-Don

AU - Reusken, Chantal B M E

AU - Koopmans, Marion P G

AU - Bosch, Berend-Jan

AU - Haagmans, Bart L

PY - 2020

Y1 - 2020

N2 - We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome.

AB - We developed and validated 2 species-independent protein-based assays to detect Middle East respiratory syndrome coronavirus functional antibodies that can block virus receptor-binding or sialic acid-attachment. Antibody levels measured in both assays correlated strongly with virus-neutralizing antibody titers, proving their use for serologic confirmatory diagnosis of Middle East respiratory syndrome.

U2 - 10.3201/eid2605.190921

DO - 10.3201/eid2605.190921

M3 - Letter

C2 - 32150528

SN - 1080-6040

VL - 26

SP - 1024

EP - 1027

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

IS - 5

ER -

Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies

Abstract

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