Separation of lactose from human milk oligosaccharides with simulated moving bed chromatography

Andreas Geisser; Tatjana Hendrich; Günther Boehm; Bernd Stahl

doi:10.1016/j.chroma.2005.03.061

Separation of lactose from human milk oligosaccharides with simulated moving bed chromatography

Andreas Geisser, Tatjana Hendrich, Günther Boehm, Bernd Stahl^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The successful separation of the disaccharide lactose from a complex mixture of human milk oligosaccharides (HMOS) with the continuous chromatography of simulated moving bed (SMB) technique is described. Since lactose is the main carbohydrate in human milk with well-known functions for the infant, it is necessary to separate it from the rest of the oligosaccharides to divide them into less complex fractions and analyse their partial unknown functions. For separation of lactose from HMOS two different stationary phases (size-exclusion gel as well as ion-exchange gel) were used. As the main result, it is shown that a size-exclusion gel with the particle size of 50-100 μm and porosity of 50 Å was the preferred stationary phase for our separation process with almost complete lactose separation and stable conditions.

Original language	English
Pages (from-to)	17-23
Number of pages	7
Journal	Journal of Chromatography A
Volume	1092
Issue number	1-2
DOIs	https://doi.org/10.1016/j.chroma.2005.03.061
Publication status	Published - 21 Oct 2005
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Mrs. Beate Müller-Werner and Mrs. Sabine Jelinek for their excellent technical assistance. Special thank goes to Mrs. Anja Frings for her reviewing the manuscript. Financial support of the Bundesministerium für Bildung und Forschung under BEO/22 0311229 is gratefully acknowledged.

Funding

We would like to thank Mrs. Beate Müller-Werner and Mrs. Sabine Jelinek for their excellent technical assistance. Special thank goes to Mrs. Anja Frings for her reviewing the manuscript. Financial support of the Bundesministerium für Bildung und Forschung under BEO/22 0311229 is gratefully acknowledged.

Keywords

Human milk oligosaccharides
Ion-exchange gel
Lactose separation
Simulated moving bed chromatography
Size-exclusion gel

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10.1016/j.chroma.2005.03.061

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title = "Separation of lactose from human milk oligosaccharides with simulated moving bed chromatography",

abstract = "The successful separation of the disaccharide lactose from a complex mixture of human milk oligosaccharides (HMOS) with the continuous chromatography of simulated moving bed (SMB) technique is described. Since lactose is the main carbohydrate in human milk with well-known functions for the infant, it is necessary to separate it from the rest of the oligosaccharides to divide them into less complex fractions and analyse their partial unknown functions. For separation of lactose from HMOS two different stationary phases (size-exclusion gel as well as ion-exchange gel) were used. As the main result, it is shown that a size-exclusion gel with the particle size of 50-100 μm and porosity of 50 {\AA} was the preferred stationary phase for our separation process with almost complete lactose separation and stable conditions.",

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author = "Andreas Geisser and Tatjana Hendrich and G{\"u}nther Boehm and Bernd Stahl",

note = "Funding Information: We would like to thank Mrs. Beate M{\"u}ller-Werner and Mrs. Sabine Jelinek for their excellent technical assistance. Special thank goes to Mrs. Anja Frings for her reviewing the manuscript. Financial support of the Bundesministerium f{\"u}r Bildung und Forschung under BEO/22 0311229 is gratefully acknowledged.",

year = "2005",

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doi = "10.1016/j.chroma.2005.03.061",

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AU - Geisser, Andreas

AU - Hendrich, Tatjana

AU - Boehm, Günther

AU - Stahl, Bernd

N1 - Funding Information: We would like to thank Mrs. Beate Müller-Werner and Mrs. Sabine Jelinek for their excellent technical assistance. Special thank goes to Mrs. Anja Frings for her reviewing the manuscript. Financial support of the Bundesministerium für Bildung und Forschung under BEO/22 0311229 is gratefully acknowledged.

PY - 2005/10/21

Y1 - 2005/10/21

N2 - The successful separation of the disaccharide lactose from a complex mixture of human milk oligosaccharides (HMOS) with the continuous chromatography of simulated moving bed (SMB) technique is described. Since lactose is the main carbohydrate in human milk with well-known functions for the infant, it is necessary to separate it from the rest of the oligosaccharides to divide them into less complex fractions and analyse their partial unknown functions. For separation of lactose from HMOS two different stationary phases (size-exclusion gel as well as ion-exchange gel) were used. As the main result, it is shown that a size-exclusion gel with the particle size of 50-100 μm and porosity of 50 Å was the preferred stationary phase for our separation process with almost complete lactose separation and stable conditions.

AB - The successful separation of the disaccharide lactose from a complex mixture of human milk oligosaccharides (HMOS) with the continuous chromatography of simulated moving bed (SMB) technique is described. Since lactose is the main carbohydrate in human milk with well-known functions for the infant, it is necessary to separate it from the rest of the oligosaccharides to divide them into less complex fractions and analyse their partial unknown functions. For separation of lactose from HMOS two different stationary phases (size-exclusion gel as well as ion-exchange gel) were used. As the main result, it is shown that a size-exclusion gel with the particle size of 50-100 μm and porosity of 50 Å was the preferred stationary phase for our separation process with almost complete lactose separation and stable conditions.

KW - Human milk oligosaccharides

KW - Ion-exchange gel

KW - Lactose separation

KW - Simulated moving bed chromatography

KW - Size-exclusion gel

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Separation of lactose from human milk oligosaccharides with simulated moving bed chromatography

Abstract

Bibliographical note

Funding

Keywords

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