Abstract
A modification of the TROSY experiment which allows for sensitivity enhancement without introduction of additional delays is presented. The method relies on the combination of two orthogonal pathways and results in a signal-to-noise gain of 2 independent of the size of the molecule. The sensitivity enhanced TROSY scheme can be combined with gradient coherence selection. The method has been applied to the 269 residue serine protease PB92. Copyright 1998 Academic Press.
| Original language | English |
|---|---|
| Pages (from-to) | 158-160 |
| Number of pages | 3 |
| Journal | Journal of Magnetic Resonance |
| Volume | 134 |
| Issue number | 1 |
| Publication status | Published - 1 Sept 1998 |
Keywords
- glycine
- serine protease PB92
- subtilisin
- article
- chemistry
- methodology
- nuclear magnetic resonance
- protein conformation
- sensitivity and specificity