Self-amplification of satellite DNA in vitro

J. B. Buntjer; J. A. Lenstra

Self-amplification of satellite DNA in vitro

J. B. Buntjer
, J. A. Lenstra^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We describe a PCR-like reaction in which genomic DNA acts as a template as well as a primer. Interaction between genomic tandem repeat units leads to self-amplification of satellite DNA. This genomic self-priming PCR (GSP-PCR) allowed the rapid amplification of species-specific tandem repeats of horse, cattle, dolphin, and chicken. A novel specific satellite of ostrich with a repeat unit of 60 bp was isolated using this method.

Original language	English
Pages (from-to)	429-434
Number of pages	6
Journal	Genome
Volume	41
Issue number	3
Publication status	Published - 1 Jun 1998

Keywords

Amplification
Isolation
Satellite DNA
Species-specific probes

Cite this

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title = "Self-amplification of satellite DNA in vitro",

abstract = "We describe a PCR-like reaction in which genomic DNA acts as a template as well as a primer. Interaction between genomic tandem repeat units leads to self-amplification of satellite DNA. This genomic self-priming PCR (GSP-PCR) allowed the rapid amplification of species-specific tandem repeats of horse, cattle, dolphin, and chicken. A novel specific satellite of ostrich with a repeat unit of 60 bp was isolated using this method.",

keywords = "Amplification, Isolation, Satellite DNA, Species-specific probes",

author = "Buntjer, \{J. B.\} and Lenstra, \{J. A.\}",

year = "1998",

month = jun,

day = "1",

language = "English",

volume = "41",

pages = "429--434",

journal = "Genome",

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publisher = "Canadian Science Publishing (Ottawa, Canada)",

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TY - JOUR

T1 - Self-amplification of satellite DNA in vitro

AU - Buntjer, J. B.

AU - Lenstra, J. A.

PY - 1998/6/1

Y1 - 1998/6/1

N2 - We describe a PCR-like reaction in which genomic DNA acts as a template as well as a primer. Interaction between genomic tandem repeat units leads to self-amplification of satellite DNA. This genomic self-priming PCR (GSP-PCR) allowed the rapid amplification of species-specific tandem repeats of horse, cattle, dolphin, and chicken. A novel specific satellite of ostrich with a repeat unit of 60 bp was isolated using this method.

AB - We describe a PCR-like reaction in which genomic DNA acts as a template as well as a primer. Interaction between genomic tandem repeat units leads to self-amplification of satellite DNA. This genomic self-priming PCR (GSP-PCR) allowed the rapid amplification of species-specific tandem repeats of horse, cattle, dolphin, and chicken. A novel specific satellite of ostrich with a repeat unit of 60 bp was isolated using this method.

KW - Amplification

KW - Isolation

KW - Satellite DNA

KW - Species-specific probes

UR - https://www.scopus.com/pages/publications/0032100198

M3 - Article

C2 - 9729777

AN - SCOPUS:0032100198

SN - 0831-2796

VL - 41

SP - 429

EP - 434

JO - Genome

JF - Genome

IS - 3

ER -

Self-amplification of satellite DNA in vitro

Abstract

Keywords

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