Abstract
Effects of the neurotoxic organic solvent toluene on human neuronal nicotinic acetylcholine (nACh) and gaminobutyric
acid type A (GABAA) neurotransmitter receptors were investigated in whole-cell voltage-clamped IMR-
32 neuroblastoma cells. Ion currents evoked by near maximum effective concentrations of 1 mM acetylcholine (ACh)
and 1 mM g-aminobutyric acid (GABA) are inhibited by toluene in a concentration-dependent way. Concentration/
effect curves of toluene yield IC50 values of 2769/26 and 399/6 mM and slope factors of 1.49/0.2 and 0.89/0.1 for
inhibition of the ACh- and GABA-induced ion currents, respectively. The results demonstrate the selective inhibition of
human GABAA receptors by toluene at concentrations comparable with brain concentrations associated with
occupational exposure.
acid type A (GABAA) neurotransmitter receptors were investigated in whole-cell voltage-clamped IMR-
32 neuroblastoma cells. Ion currents evoked by near maximum effective concentrations of 1 mM acetylcholine (ACh)
and 1 mM g-aminobutyric acid (GABA) are inhibited by toluene in a concentration-dependent way. Concentration/
effect curves of toluene yield IC50 values of 2769/26 and 399/6 mM and slope factors of 1.49/0.2 and 0.89/0.1 for
inhibition of the ACh- and GABA-induced ion currents, respectively. The results demonstrate the selective inhibition of
human GABAA receptors by toluene at concentrations comparable with brain concentrations associated with
occupational exposure.
Original language | Undefined/Unknown |
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Pages (from-to) | 243-248 |
Number of pages | 6 |
Journal | Toxicology |
Volume | 190 |
Publication status | Published - 2003 |
Keywords
- Diergeneeskunde (DGNK)