Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I

Ngalle Eric Mbua; Xiuru Li; Heather R Flanagan-Steet; Lu Meng; Kazuhiro Aoki; Kelley W Moremen; Margreet A Wolfert; Richard Steet; Geert-Jan Boons

doi:10.1002/anie.201307095

Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I

Ngalle Eric Mbua, Xiuru Li, Heather R Flanagan-Steet, Lu Meng, Kazuhiro Aoki, Kelley W Moremen, Margreet A Wolfert, Richard Steet, Geert-Jan Boons

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Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease.

Original language	English
Pages (from-to)	13012-5
Number of pages	4
Journal	Angewandte Chemie-International Edition
Volume	52
Issue number	49
DOIs	https://doi.org/10.1002/anie.201307095
Publication status	Published - 2 Dec 2013
Externally published	Yes

Keywords

Click Chemistry
Fibroblasts
Humans
N-Acetylneuraminic Acid
Polysaccharides
Recombinant Proteins
Sialic Acids
Sialyltransferases

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title = "Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I",

abstract = "A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease.",

keywords = "Click Chemistry, Fibroblasts, Humans, N-Acetylneuraminic Acid, Polysaccharides, Recombinant Proteins, Sialic Acids, Sialyltransferases",

author = "Mbua, {Ngalle Eric} and Xiuru Li and Flanagan-Steet, {Heather R} and Lu Meng and Kazuhiro Aoki and Moremen, {Kelley W} and Wolfert, {Margreet A} and Richard Steet and Geert-Jan Boons",

note = "Copyright {\textcopyright} 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2013",

month = dec,

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doi = "10.1002/anie.201307095",

language = "English",

volume = "52",

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journal = "Angewandte Chemie-International Edition",

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publisher = "John Wiley and Sons Ltd",

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T1 - Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I

AU - Mbua, Ngalle Eric

AU - Li, Xiuru

AU - Flanagan-Steet, Heather R

AU - Meng, Lu

AU - Aoki, Kazuhiro

AU - Moremen, Kelley W

AU - Wolfert, Margreet A

AU - Steet, Richard

AU - Boons, Geert-Jan

PY - 2013/12/2

Y1 - 2013/12/2

N2 - A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease.

AB - A game of tag: N-Glycans on the surface of living cells were selectively tagged by exogenously administering recombinant ST6Gal I sialyltransferase and azide-modified CMP-Neu5Ac. This modification was followed by a strain-promoted cycloaddition using a biotin-modified dibenzylcyclooctynol (red star=biotin). The methodology will make it possible to dissect the mechanisms that underlie altered glycoconjugate recycling and storage in disease.

KW - Click Chemistry

KW - Fibroblasts

KW - Humans

KW - N-Acetylneuraminic Acid

KW - Polysaccharides

KW - Recombinant Proteins

KW - Sialic Acids

KW - Sialyltransferases

U2 - 10.1002/anie.201307095

DO - 10.1002/anie.201307095

M3 - Article

C2 - 24129959

SN - 1433-7851

VL - 52

SP - 13012

EP - 13015

JO - Angewandte Chemie-International Edition

JF - Angewandte Chemie-International Edition

IS - 49

ER -

Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I

Abstract

Keywords

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