Secretome Analysis: Reading Cellular Sign Language to Understand Intercellular Communication

Wei Wu; Jeroen Krijgsveld

doi:10.1016/j.mcpro.2023.100692

Secretome Analysis: Reading Cellular Sign Language to Understand Intercellular Communication

Wei Wu
, Jeroen Krijgsveld

extern

Research output: Contribution to journal › Review article › peer-review

Abstract

A significant portion of mammalian proteomes is secreted to the extracellular space to fulfill crucial roles in cell-to-cell communication. To best recapitulate the intricate and multi-faceted crosstalk between cells in a live organism, there is an ever-increasing need for methods to study protein secretion in model systems that include multiple cell types. In addition, posttranslational modifications further expand the complexity and versatility of cellular communication. This review aims to summarize recent strategies and model systems that employ cellular coculture, chemical biology tools, protein enrichment, and proteomic methods to characterize the composition and function of cellular secretomes. This is all geared towards gaining better understanding of organismal biology in vivo mediated by secretory signaling.

Original language	English
Article number	100692
Journal	Molecular and Cellular Proteomics
Volume	23
Issue number	1
DOIs	https://doi.org/10.1016/j.mcpro.2023.100692
Publication status	Published - Jan 2024
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2023 THE AUTHORS.

Funding

W. W. is supported by Singapore Immunology Network (SIgN) , Agency for Science, Technology and Research (A*STAR) ; Biomedical Research Council (BMRC) Core Research Fund for Use-Inspired Basic research (UIBR) and IAF-PP project H22J2a0043, and Singapore National Medical Research Council (NMRC) project MOH-001401-00. J. K. is supported by the Deutsche Forschungsgemeinschaft (DFG) , SFB 1324; by the Deutsche Krebshilfe, project 70114190; and by the German Ministry of Education and Research (BMBF) , as part of the National Research Node "Mass Spectrometry in Systems Medicine" (MSCoreSys) , project 161L0212A.

Funders	Funder number
Singapore Immunology Network (SIgN) , Agency for Science, Technology and Research (A*STAR)
Biomedical Research Council (BMRC) Core Research Fund for use-inspired basic research (UIBR)
IAF-PP project	H22J2a0043
Singapore National Medical Research Council (NMRC)	MOH-001401-00
Deutsche Forschungsgemeinschaft (DFG)	SFB 1324
Deutsche Krebshilfe	70114190
German Ministry of Education and Research (BMBF) , as part of the National Research Node "Mass Spectrometry in Systems Medicine" (MSCoreSys)	161L0212A

Keywords

Animals
Cell Communication
Mammals/metabolism
Proteome/metabolism
Proteomics/methods
Secretome

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10.1016/j.mcpro.2023.100692

Cite this

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title = "Secretome Analysis: Reading Cellular Sign Language to Understand Intercellular Communication",

abstract = "A significant portion of mammalian proteomes is secreted to the extracellular space to fulfill crucial roles in cell-to-cell communication. To best recapitulate the intricate and multi-faceted crosstalk between cells in a live organism, there is an ever-increasing need for methods to study protein secretion in model systems that include multiple cell types. In addition, posttranslational modifications further expand the complexity and versatility of cellular communication. This review aims to summarize recent strategies and model systems that employ cellular coculture, chemical biology tools, protein enrichment, and proteomic methods to characterize the composition and function of cellular secretomes. This is all geared towards gaining better understanding of organismal biology in vivo mediated by secretory signaling.",

keywords = "Animals, Cell Communication, Mammals/metabolism, Proteome/metabolism, Proteomics/methods, Secretome",

author = "Wei Wu and Jeroen Krijgsveld",

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year = "2024",

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doi = "10.1016/j.mcpro.2023.100692",

language = "English",

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AB - A significant portion of mammalian proteomes is secreted to the extracellular space to fulfill crucial roles in cell-to-cell communication. To best recapitulate the intricate and multi-faceted crosstalk between cells in a live organism, there is an ever-increasing need for methods to study protein secretion in model systems that include multiple cell types. In addition, posttranslational modifications further expand the complexity and versatility of cellular communication. This review aims to summarize recent strategies and model systems that employ cellular coculture, chemical biology tools, protein enrichment, and proteomic methods to characterize the composition and function of cellular secretomes. This is all geared towards gaining better understanding of organismal biology in vivo mediated by secretory signaling.

KW - Animals

KW - Cell Communication

KW - Mammals/metabolism

KW - Proteome/metabolism

KW - Proteomics/methods

KW - Secretome

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DO - 10.1016/j.mcpro.2023.100692

M3 - Review article

C2 - 38081362

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VL - 23

JO - Molecular and Cellular Proteomics

JF - Molecular and Cellular Proteomics

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ER -

Secretome Analysis: Reading Cellular Sign Language to Understand Intercellular Communication

Abstract

Bibliographical note

Funding

Keywords

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