Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host

Michiel L Bexkens; Renske A van Gestel; Bas van Breukelen; Rolf T Urbanus; Jos F Brouwers; Rienk Nieuwland; Aloysius G M Tielens; Jaap J van Hellemond

doi:10.1016/j.molbiopara.2020.111296

Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host

Michiel L Bexkens, Renske A van Gestel, Bas van Breukelen, Rolf T Urbanus, Jos F Brouwers, Rienk Nieuwland, Aloysius G M Tielens, Jaap J van Hellemond

Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands; Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Wytemaweg 80, 3015 CE Rotterdam, The Netherlands.
Department of Clinical Chemistry and Haematology, Center for Circulatory Health, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.
Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands; Vesicle Observation Centre, Amsterdam UMC, University of Amsterdam, Amsterdam, the Netherlands.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Eggs, schistosomula and adult Schistosoma worms are known to release extracellular vesicles (EV) during in vitro incubations and these EVs are postulated to affect the host responses. So far only those EVs released during in vitro incubations of schistosomes have been studied and it is unknown whether in blood of infected hosts the schistosomal EVs can be detected amidst all the circulating EVs of the host itself. In this study we analyzed the protein as well as the phospholipid composition of EVs circulating in blood plasma of S. mansoni infected hamsters and compared those with the EVs circulating in blood of non-infected hamsters. Although neither proteins nor lipids specific for schistosomes could be detected in the circulating EVs of the infected hamsters, the infection with schistosomes had a marked effect on the circulating EVs of the host, as the protein as well as the lipid composition of EVs circulating in infected hamsters were different from the EVs of uninfected hamsters. The observed changes in the EV lipid and protein content suggest that more EVs are released by the diseased liver, the affected erythrocytes and activated immune cells.

Original language	English
Article number	111296
Number of pages	7
Journal	Molecular and Biochemical Parasitology
Volume	238
DOIs	https://doi.org/10.1016/j.molbiopara.2020.111296
Publication status	Published - Jul 2020

Keywords

Exosomes
Cell-derived microparticles
Schistosomiasis
Host-parasite interactions
Communicable diseases

Access to Document

10.1016/j.molbiopara.2020.111296

1-s2.0-S0166685120300608-mainFinal published version, 1.84 MBLicence: CC BY

Cite this

Bexkens, M. L., van Gestel, R. A., van Breukelen, B., Urbanus, R. T., Brouwers, J. F., Nieuwland, R., Tielens, A. G. M., & van Hellemond, J. J. (2020). Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host. Molecular and Biochemical Parasitology, 238, Article 111296. https://doi.org/10.1016/j.molbiopara.2020.111296

@article{269154191f24454190f8a85615b5d649,

title = "Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host",

abstract = "Eggs, schistosomula and adult Schistosoma worms are known to release extracellular vesicles (EV) during in vitro incubations and these EVs are postulated to affect the host responses. So far only those EVs released during in vitro incubations of schistosomes have been studied and it is unknown whether in blood of infected hosts the schistosomal EVs can be detected amidst all the circulating EVs of the host itself. In this study we analyzed the protein as well as the phospholipid composition of EVs circulating in blood plasma of S. mansoni infected hamsters and compared those with the EVs circulating in blood of non-infected hamsters. Although neither proteins nor lipids specific for schistosomes could be detected in the circulating EVs of the infected hamsters, the infection with schistosomes had a marked effect on the circulating EVs of the host, as the protein as well as the lipid composition of EVs circulating in infected hamsters were different from the EVs of uninfected hamsters. The observed changes in the EV lipid and protein content suggest that more EVs are released by the diseased liver, the affected erythrocytes and activated immune cells.",

keywords = "Exosomes, Cell-derived microparticles, Schistosomiasis, Host-parasite interactions, Communicable diseases",

author = "Bexkens, {Michiel L} and {van Gestel}, {Renske A} and {van Breukelen}, Bas and Urbanus, {Rolf T} and Brouwers, {Jos F} and Rienk Nieuwland and Tielens, {Aloysius G M} and {van Hellemond}, {Jaap J}",

year = "2020",

month = jul,

doi = "10.1016/j.molbiopara.2020.111296",

language = "English",

volume = "238",

journal = "Molecular and Biochemical Parasitology",

issn = "0166-6851",

publisher = "Elsevier",

}

TY - JOUR

T1 - Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host

AU - Bexkens, Michiel L

AU - van Gestel, Renske A

AU - van Breukelen, Bas

AU - Urbanus, Rolf T

AU - Brouwers, Jos F

AU - Nieuwland, Rienk

AU - Tielens, Aloysius G M

AU - van Hellemond, Jaap J

PY - 2020/7

Y1 - 2020/7

N2 - Eggs, schistosomula and adult Schistosoma worms are known to release extracellular vesicles (EV) during in vitro incubations and these EVs are postulated to affect the host responses. So far only those EVs released during in vitro incubations of schistosomes have been studied and it is unknown whether in blood of infected hosts the schistosomal EVs can be detected amidst all the circulating EVs of the host itself. In this study we analyzed the protein as well as the phospholipid composition of EVs circulating in blood plasma of S. mansoni infected hamsters and compared those with the EVs circulating in blood of non-infected hamsters. Although neither proteins nor lipids specific for schistosomes could be detected in the circulating EVs of the infected hamsters, the infection with schistosomes had a marked effect on the circulating EVs of the host, as the protein as well as the lipid composition of EVs circulating in infected hamsters were different from the EVs of uninfected hamsters. The observed changes in the EV lipid and protein content suggest that more EVs are released by the diseased liver, the affected erythrocytes and activated immune cells.

AB - Eggs, schistosomula and adult Schistosoma worms are known to release extracellular vesicles (EV) during in vitro incubations and these EVs are postulated to affect the host responses. So far only those EVs released during in vitro incubations of schistosomes have been studied and it is unknown whether in blood of infected hosts the schistosomal EVs can be detected amidst all the circulating EVs of the host itself. In this study we analyzed the protein as well as the phospholipid composition of EVs circulating in blood plasma of S. mansoni infected hamsters and compared those with the EVs circulating in blood of non-infected hamsters. Although neither proteins nor lipids specific for schistosomes could be detected in the circulating EVs of the infected hamsters, the infection with schistosomes had a marked effect on the circulating EVs of the host, as the protein as well as the lipid composition of EVs circulating in infected hamsters were different from the EVs of uninfected hamsters. The observed changes in the EV lipid and protein content suggest that more EVs are released by the diseased liver, the affected erythrocytes and activated immune cells.

KW - Exosomes

KW - Cell-derived microparticles

KW - Schistosomiasis

KW - Host-parasite interactions

KW - Communicable diseases

U2 - 10.1016/j.molbiopara.2020.111296

DO - 10.1016/j.molbiopara.2020.111296

M3 - Article

C2 - 32603736

SN - 0166-6851

VL - 238

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

M1 - 111296

ER -

Schistosoma mansoni infection affects the proteome and lipidome of circulating extracellular vesicles in the host

Abstract

Keywords

Access to Document

Fingerprint

Cite this