Sarcosin (Krp1) in skeletal muscle differentiation: gene expression profiling and knockdown experiments

Leonie du Puy; Abdelaziz Beqqali; Helena T A van Tol; Jantine Monshouwer-Kloots; Robert Passier; Henk P Haagsman; Bernard A J Roelen

doi:10.1387/ijdb.113327lp

Sarcosin (Krp1) in skeletal muscle differentiation: gene expression profiling and knockdown experiments

Leonie du Puy, Abdelaziz Beqqali, Helena T A van Tol, Jantine Monshouwer-Kloots, Robert Passier, Henk P Haagsman, Bernard A J Roelen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

SARCOSIN, also named Krp1, has been identified as a protein exclusively expressed in striated muscle tissue. Here we report on the role of SARCOSIN in skeletal muscle development and differentiation. We demonstrate, by means of whole-mount in situ hybridization, that Sarcosin mRNA is expressed in the myotome part of the mature somites in mouse embryos from embryonic day 9.5 onwards. Sarcosin is not expressed in the developing heart at these embryonic stages, and in adult tissues the mRNA expression levels are five times lower in the heart than in skeletal muscle. SARCOSIN protein partially co-localizes with the M-band protein myomesin and between and below laterally fusing myofibrils in adult skeletal muscle tissue. RNA interference mediated knock-down of SARCOSIN in the C2C12 myoblast cell line appeared to be stimulatory in the early phase of differentiation, but inhibitory at a later phase of differentiation.

Original language	English
Pages (from-to)	301-9
Number of pages	9
Journal	International Journal of Developmental Biology
Volume	56
Issue number	4
DOIs	https://doi.org/10.1387/ijdb.113327lp
Publication status	Published - 2012

Keywords

Animals
Cell Differentiation
Cell Line
Connectin
Embryo, Mammalian
Female
Gene Expression Profiling
Gene Expression Regulation, Developmental
Immunoblotting
In Situ Hybridization
Male
Mice
Mice, Inbred C57BL
Microscopy, Fluorescence
Muscle Development
Muscle Fibers, Skeletal
Muscle Proteins
Muscle, Skeletal
Myoblasts
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
Somites
Time Factors

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10.1387/ijdb.113327lp

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title = "Sarcosin (Krp1) in skeletal muscle differentiation: gene expression profiling and knockdown experiments",

abstract = "SARCOSIN, also named Krp1, has been identified as a protein exclusively expressed in striated muscle tissue. Here we report on the role of SARCOSIN in skeletal muscle development and differentiation. We demonstrate, by means of whole-mount in situ hybridization, that Sarcosin mRNA is expressed in the myotome part of the mature somites in mouse embryos from embryonic day 9.5 onwards. Sarcosin is not expressed in the developing heart at these embryonic stages, and in adult tissues the mRNA expression levels are five times lower in the heart than in skeletal muscle. SARCOSIN protein partially co-localizes with the M-band protein myomesin and between and below laterally fusing myofibrils in adult skeletal muscle tissue. RNA interference mediated knock-down of SARCOSIN in the C2C12 myoblast cell line appeared to be stimulatory in the early phase of differentiation, but inhibitory at a later phase of differentiation.",

keywords = "Animals, Cell Differentiation, Cell Line, Connectin, Embryo, Mammalian, Female, Gene Expression Profiling, Gene Expression Regulation, Developmental, Immunoblotting, In Situ Hybridization, Male, Mice, Mice, Inbred C57BL, Microscopy, Fluorescence, Muscle Development, Muscle Fibers, Skeletal, Muscle Proteins, Muscle, Skeletal, Myoblasts, RNA Interference, Reverse Transcriptase Polymerase Chain Reaction, Somites, Time Factors",

author = "{du Puy}, Leonie and Abdelaziz Beqqali and {van Tol}, {Helena T A} and Jantine Monshouwer-Kloots and Robert Passier and Haagsman, {Henk P} and Roelen, {Bernard A J}",

year = "2012",

doi = "10.1387/ijdb.113327lp",

language = "English",

volume = "56",

pages = "301--9",

journal = "International Journal of Developmental Biology",

issn = "0214-6282",

publisher = "UPV/EHU Press",

number = "4",

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TY - JOUR

T1 - Sarcosin (Krp1) in skeletal muscle differentiation

T2 - gene expression profiling and knockdown experiments

AU - du Puy, Leonie

AU - Beqqali, Abdelaziz

AU - van Tol, Helena T A

AU - Monshouwer-Kloots, Jantine

AU - Passier, Robert

AU - Haagsman, Henk P

AU - Roelen, Bernard A J

PY - 2012

Y1 - 2012

N2 - SARCOSIN, also named Krp1, has been identified as a protein exclusively expressed in striated muscle tissue. Here we report on the role of SARCOSIN in skeletal muscle development and differentiation. We demonstrate, by means of whole-mount in situ hybridization, that Sarcosin mRNA is expressed in the myotome part of the mature somites in mouse embryos from embryonic day 9.5 onwards. Sarcosin is not expressed in the developing heart at these embryonic stages, and in adult tissues the mRNA expression levels are five times lower in the heart than in skeletal muscle. SARCOSIN protein partially co-localizes with the M-band protein myomesin and between and below laterally fusing myofibrils in adult skeletal muscle tissue. RNA interference mediated knock-down of SARCOSIN in the C2C12 myoblast cell line appeared to be stimulatory in the early phase of differentiation, but inhibitory at a later phase of differentiation.

AB - SARCOSIN, also named Krp1, has been identified as a protein exclusively expressed in striated muscle tissue. Here we report on the role of SARCOSIN in skeletal muscle development and differentiation. We demonstrate, by means of whole-mount in situ hybridization, that Sarcosin mRNA is expressed in the myotome part of the mature somites in mouse embryos from embryonic day 9.5 onwards. Sarcosin is not expressed in the developing heart at these embryonic stages, and in adult tissues the mRNA expression levels are five times lower in the heart than in skeletal muscle. SARCOSIN protein partially co-localizes with the M-band protein myomesin and between and below laterally fusing myofibrils in adult skeletal muscle tissue. RNA interference mediated knock-down of SARCOSIN in the C2C12 myoblast cell line appeared to be stimulatory in the early phase of differentiation, but inhibitory at a later phase of differentiation.

KW - Animals

KW - Cell Differentiation

KW - Cell Line

KW - Connectin

KW - Embryo, Mammalian

KW - Female

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Developmental

KW - Immunoblotting

KW - In Situ Hybridization

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Microscopy, Fluorescence

KW - Muscle Development

KW - Muscle Fibers, Skeletal

KW - Muscle Proteins

KW - Muscle, Skeletal

KW - Myoblasts

KW - RNA Interference

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Somites

KW - Time Factors

U2 - 10.1387/ijdb.113327lp

DO - 10.1387/ijdb.113327lp

M3 - Article

C2 - 22562206

SN - 0214-6282

VL - 56

SP - 301

EP - 309

JO - International Journal of Developmental Biology

JF - International Journal of Developmental Biology

IS - 4

ER -

Sarcosin (Krp1) in skeletal muscle differentiation: gene expression profiling and knockdown experiments

Abstract

Keywords

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