Abstract
The present study examines the hypothesis that during depletion of high- energy phosphates a change will occur in the phospholipid topology and in Ca fluxes in cultured neonatal cells and that these two events may be causally related. A combination of 2-deoxyglucose and iodoacetic acid was used to produce graded changes in the adenine nucleotides in the cells. An on-line technique for 45Ca measurement was used to follow Ca uptake and compartmentation by the cells, and chemical and enzymatic probes were used to study sarcolemmal phospholipid topology. After 5 min of metabolic inhibition (ATP = 10% of control) an increase in cellular Ca occurs, which progresses with time. Over 70% of this Ca accumulates in the mitochondria. After 30 min of metabolic inhibition (ATP
| Original language | English |
|---|---|
| Journal | American Journal of Physiology |
| Volume | 265 |
| Issue number | 2 34-2 |
| Publication status | Published - 28 Jan 1993 |
Keywords
- calcium fluxes
- adenosine triphosphate
- calcium 45
- cardiolipin
- deoxyglucose
- iodoacetic acid
- lactate dehydrogenase
- nicotinamide adenine dinucleotide
- phosphatidylcholine
- phosphatidylethanolamine
- phosphatidylinositol
- phosphatidylserine
- phospholipase A2
- phospholipid
- reduced nicotinamide adenine dinucleotide
- sphingomyelin
- trinitrobenzenesulfonic acid
- animal cell
- article
- calcium cell level
- calcium transport
- cellular distribution
- controlled study
- extracellular calcium
- cardiac muscle cell
- heart muscle fiber membrane
- high energy phosphate
- metabolic inhibition
- newborn
- nonhuman
- priority journal
- rat