Robust immune responses elicited by a fully synthetic three-component vaccine

Sampat Ingale, Margreet A. Wolfert, Jidnyasa Gaekwad, Therese Buskas, Geert-Jan Boons

Research output: Contribution to journalArticleAcademicpeer-review


The overexpression of saccharides such as Globo-H, LewisY and Tn antigen is a common feature of oncogenic transformed cells. Endeavors to exploit this aberrant glycosylation for cancer vaccine development have been complicated by difficulties in eliciting high titers of IgG antibodies against classical conjugates of tumor-associated carbohydrates to carrier proteins. We have designed, chemically synthesized and immunologically evaluated a number of fully synthetic vaccine candidates to establish strategies to overcome the poor immunogenicity of tumor-associated carbohydrates and glycopeptides. We have found that a three-component vaccine composed of a TLR2 agonist, a promiscuous peptide T-helper epitope and a tumor-associated glycopeptide can elicit in mice exceptionally high titers of IgG antibodies that can recognize cancer cells expressing the tumor-associated carbohydrate. The superior properties of the vaccine candidate are attributed to the local production of cytokines, upregulation of co-stimulatory proteins, enhanced uptake by macrophages and dendritic cells and avoidance of epitope suppression. © 2007 Nature Publishing Group.
Original languageEnglish
Pages (from-to)663-667
Number of pages5
JournalNature Chemical Biology
Issue number10
Publication statusPublished - 20 Oct 2007
Externally publishedYes


  • cancer vaccine
  • epitope
  • glycopeptide
  • immunoglobulin G
  • immunoglobulin G1
  • immunoglobulin G2a
  • immunoglobulin G2b
  • immunoglobulin G3
  • immunoglobulin M
  • immunological adjuvant
  • liposome
  • mucin 1 antibody
  • protein antibody
  • qs 21
  • toll like receptor 2 agonist
  • unclassified drug
  • animal cell
  • animal experiment
  • animal model
  • antibody titer
  • antigen presentation
  • article
  • controlled study
  • cytokine production
  • dendritic cell
  • female
  • flow cytometry
  • human
  • human cell
  • immune response
  • immunogenicity
  • immunoglobulin blood level
  • macrophage
  • mouse
  • nonhuman
  • priority journal
  • upregulation


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