(R)evolution-on-a-chip

Evgenios Bouzetos; Ketan Ashok Ganar; Enrico Mastrobattista; Siddharth Deshpande; John van der Oost

doi:10.1016/j.tibtech.2021.04.009

(R)evolution-on-a-chip

Evgenios Bouzetos, Ketan Ashok Ganar, Enrico Mastrobattista, Siddharth Deshpande^*, John van der Oost

^*Corresponding author for this work

Wageningen University & Research

Research output: Contribution to journal › Review article › peer-review

Abstract

Billions of years of Darwinian evolution has led to the emergence of highly sophisticated and diverse life forms on Earth. Inspired by natural evolution, similar principles have been adopted in laboratory evolution for the fast optimization of genes and proteins for specific applications. In this review, we highlight state-of-the-art laboratory evolution strategies for protein engineering, with a special emphasis on in vitro strategies. We further describe how recent progress in microfluidic technology has allowed the generation and manipulation of artificial compartments for high-throughput laboratory evolution experiments. Expectations for the future are high: we foresee a revolution on-a-chip.

Original language	English
Pages (from-to)	60-76
Number of pages	17
Journal	Trends in Biotechnology
Volume	40
Issue number	1
DOIs	https://doi.org/10.1016/j.tibtech.2021.04.009
Publication status	Published - Jan 2022

Bibliographical note

Funding Information:
We are very grateful to the reviewers for their constructive feedback. One of them revealed his identity: Dan Tawfik. A few weeks after the revised version was accepted for publication, we were shocked to learn that Dan tragically passed away. We would like to dedicate this review article to Dan Tawfik for important contributions in the field of in vitro evolution. The authors acknowledge financial support by the Innovation Program Microbiology of Wageningen University (IPM-3 grant 6145010520 ) to S.D. and by the European Research Council (ERC AdG 2018-834279 ) to J.V.D.O. The authors would also like to thank Vivaan Deshpande for helping to acquire the ink-in-water image.

Publisher Copyright:
© 2021 The Author(s)

Funding

We are very grateful to the reviewers for their constructive feedback. One of them revealed his identity: Dan Tawfik. A few weeks after the revised version was accepted for publication, we were shocked to learn that Dan tragically passed away. We would like to dedicate this review article to Dan Tawfik for important contributions in the field of in vitro evolution. The authors acknowledge financial support by the Innovation Program Microbiology of Wageningen University (IPM-3 grant 6145010520 ) to S.D. and by the European Research Council (ERC AdG 2018-834279 ) to J.V.D.O. The authors would also like to thank Vivaan Deshpande for helping to acquire the ink-in-water image.

Keywords

biocatalyst
emulsion
in vitro compartmentalization
laboratory evolution
microfluidics
vesicle

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10.1016/j.tibtech.2021.04.009Licence: CC BY

1-s2.0-S0167779921001074-mainFinal published version, 3.26 MBLicence: CC BY

Cite this

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abstract = "Billions of years of Darwinian evolution has led to the emergence of highly sophisticated and diverse life forms on Earth. Inspired by natural evolution, similar principles have been adopted in laboratory evolution for the fast optimization of genes and proteins for specific applications. In this review, we highlight state-of-the-art laboratory evolution strategies for protein engineering, with a special emphasis on in vitro strategies. We further describe how recent progress in microfluidic technology has allowed the generation and manipulation of artificial compartments for high-throughput laboratory evolution experiments. Expectations for the future are high: we foresee a revolution on-a-chip.",

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AU - van der Oost, John

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(R)evolution-on-a-chip

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