TY - JOUR
T1 - Responses of Brassica oleracea cultivars to infestation by the aphid Brevicoryne brassicae
T2 - An ecological and molecular approach
AU - Broekgaarden, Colette
AU - Poelman, Erik H.
AU - Steenhuis, Greet
AU - Voorrips, Roeland E.
AU - Dicke, Marcel
AU - Vosman, Ben
PY - 2008/11/1
Y1 - 2008/11/1
N2 - Intraspecific variation in resistance or susceptibility to herbivorous insects has been widely studied through bioassays. However, few studies have combined this with a full transcriptomic analysis. Here, we take such an approach to study the interaction between the aphid Brevicoryne brassicae and four white cabbage (Brassica oleracea var. capitata) cultivars. Both under glasshouse and field conditions, two of the cultivars clearly supported a faster aphid population development than the other two, indicating that aphid population development was largely independent of the environmental conditions. Genome-wide transcriptomic analysis using 70-mer oligonucleotide microarrays based on the Arabidopsis thaliana genome showed that only a small number of genes were differentially regulated, and that this regulation was highly cultivar specific. The temporal pattern in the expression behaviour of two B. brassicae-responsive genes in all four cultivars together with targeted studies employing A. thaliana knockout mutants revealed a possible role for a trypsin-and-protease inhibitor in defence against B. brassicae. Conversely, a xyloglucan endotransglucosylase seemed to have no effect on aphid performance. Overall, this study shows clear intraspecific variation in B. brassicae susceptibility among B. oleracea cultivars under glasshouse and field conditions that can be partly explained by certain differences in induced transcriptional changes.
AB - Intraspecific variation in resistance or susceptibility to herbivorous insects has been widely studied through bioassays. However, few studies have combined this with a full transcriptomic analysis. Here, we take such an approach to study the interaction between the aphid Brevicoryne brassicae and four white cabbage (Brassica oleracea var. capitata) cultivars. Both under glasshouse and field conditions, two of the cultivars clearly supported a faster aphid population development than the other two, indicating that aphid population development was largely independent of the environmental conditions. Genome-wide transcriptomic analysis using 70-mer oligonucleotide microarrays based on the Arabidopsis thaliana genome showed that only a small number of genes were differentially regulated, and that this regulation was highly cultivar specific. The temporal pattern in the expression behaviour of two B. brassicae-responsive genes in all four cultivars together with targeted studies employing A. thaliana knockout mutants revealed a possible role for a trypsin-and-protease inhibitor in defence against B. brassicae. Conversely, a xyloglucan endotransglucosylase seemed to have no effect on aphid performance. Overall, this study shows clear intraspecific variation in B. brassicae susceptibility among B. oleracea cultivars under glasshouse and field conditions that can be partly explained by certain differences in induced transcriptional changes.
KW - Aphid performance
KW - Brassica oleracea
KW - Brevicoryne brassicae
KW - Comparative genomics
KW - Defence
KW - Gene expression
KW - Microarray
UR - http://www.scopus.com/inward/record.url?scp=53349171733&partnerID=8YFLogxK
U2 - 10.1111/j.1365-3040.2008.01871.x
DO - 10.1111/j.1365-3040.2008.01871.x
M3 - Article
C2 - 18721268
AN - SCOPUS:53349171733
SN - 0140-7791
VL - 31
SP - 1592
EP - 1605
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
IS - 11
ER -