Reporters for sensitive and quantitative measurement of auxin response

Che Yang Liao; Wouter Smet; Geraldine Brunoud; Saiko Yoshida; Teva Vernoux; Dolf Weijers

doi:10.1038/nmeth.3279

Reporters for sensitive and quantitative measurement of auxin response

Che Yang Liao
, Wouter Smet
, Geraldine Brunoud
, Saiko Yoshida
, Teva Vernoux
, Dolf Weijers^*

^*Corresponding author for this work

Wageningen University & Research
École normale supérieure de Lyon
Institute of Science and Technology Austria
extern

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The visualization of hormonal signaling input and output is key to understanding how multicellular development is regulated. The plant signaling molecule auxin triggers many growth and developmental responses, but current tools lack the sensitivity or precision to visualize these. We developed a set of fluorescent reporters that allow sensitive and semiquantitative readout of auxin responses at cellular resolution in Arabidopsis thaliana. These generic tools are suitable for any transformable plant species.

Original language	English
Pages (from-to)	207-210
Number of pages	4
Journal	Nature Methods
Volume	12
Issue number	3
DOIs	https://doi.org/10.1038/nmeth.3279
Publication status	Published - 26 Feb 2015
Externally published	Yes

Bibliographical note

Funding Information:
Netherlands Organization for Scientific Research (NWO; ALW-820.02.019) to D.W. and Human Frontier Science Program (HFSP; research grant RGP0054-2013) and Agence Nationale de la Recherche (ANR; AuxiFlo; grant ANR-12-BSV6-0005) to T.V.

Funding Information:
We thank T. Laux (Universität Freiburg) for plasmids and B. de Rybel for helpful comments on the manuscript. This work was supported by grants from the European Research Council (ERC; CELLPATTERN; contract number 281573) and the

Publisher Copyright:
© 2015 Nature America, Inc.

Funding

Netherlands Organization for Scientific Research (NWO; ALW-820.02.019) to D.W. and Human Frontier Science Program (HFSP; research grant RGP0054-2013) and Agence Nationale de la Recherche (ANR; AuxiFlo; grant ANR-12-BSV6-0005) to T.V. We thank T. Laux (Universität Freiburg) for plasmids and B. de Rybel for helpful comments on the manuscript. This work was supported by grants from the European Research Council (ERC; CELLPATTERN; contract number 281573) and the

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10.1038/nmeth.3279

Cite this

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AU - Weijers, Dolf

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Reporters for sensitive and quantitative measurement of auxin response

Abstract

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