Repeated exposure of bronchial epithelial cells to particular matter increases allergen-induced cytokine release and permeability

Hamed Janbazacyabar; Jeroen van Bergenhenegouwen; Soheil Varasteh; Johan Garssen; Gert Folkerts; Saskia Braber

doi:10.1016/j.cyto.2022.155878

Repeated exposure of bronchial epithelial cells to particular matter increases allergen-induced cytokine release and permeability

Hamed Janbazacyabar
, Jeroen van Bergenhenegouwen
, Soheil Varasteh
, Johan Garssen
, Gert Folkerts
, Saskia Braber

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Long term particulate matter (PM) exposure has been associated with an increased incidence of respiratory diseases. Here, an in vitro model was developed to study how long term diesel exhaust particle (DEP) exposure might predispose to the development of allergic reactions. Airway epithelial (16HBE) cells were exposed to low concentrations of diesel exhaust particle (DEP) for 4 days after which they were challenged with house dust mite (HDM) extract (24 h). Compared to acute exposure (24 h), 4 days DEP exposure to 16HBE cells further reduced the transepithelial electrical resistance (TEER) and increased CXCL-8 release. DEP pre-exposure aggravated HDM-induced loss of TEER, increased tracer flux across the barrier and reduced CLDN-3 expression in these 16HBE cells. HDM-induced cytokine (IL-6, CCL-22, IL-10 and CXCL-8) release was significantly increased after DEP pre-exposure. In the current study an in vitro model with long term PM exposure was presented, which might be helpful for further understanding the interplay between long term PM exposure and allergic responses.

Original language	English
Article number	155878
Pages (from-to)	1-11
Journal	Cytokine
Volume	154
Early online date	8 Apr 2022
DOIs	https://doi.org/10.1016/j.cyto.2022.155878
Publication status	Published - Jun 2022

Bibliographical note

Funding Information:
The authors would like to thank Mies van Steenbergen for his technical support related to diesel exhaust particles preparation and size examination. Conflict of Interest: JvB and JG are employees of DNR. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Funding Source: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Publisher Copyright:
© 2022 The Authors

Keywords

16HBE cells
Air pollution
Airway epithelial cells
Allergy
Chronic
House dust mite
In vitro, Immune responses
Particulate matter

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1-s2.0-S1043466622000874-mainFinal published version, 3.87 MBLicence: CC BY

Cite this

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title = "Repeated exposure of bronchial epithelial cells to particular matter increases allergen-induced cytokine release and permeability",

abstract = "Long term particulate matter (PM) exposure has been associated with an increased incidence of respiratory diseases. Here, an in vitro model was developed to study how long term diesel exhaust particle (DEP) exposure might predispose to the development of allergic reactions. Airway epithelial (16HBE) cells were exposed to low concentrations of diesel exhaust particle (DEP) for 4 days after which they were challenged with house dust mite (HDM) extract (24 h). Compared to acute exposure (24 h), 4 days DEP exposure to 16HBE cells further reduced the transepithelial electrical resistance (TEER) and increased CXCL-8 release. DEP pre-exposure aggravated HDM-induced loss of TEER, increased tracer flux across the barrier and reduced CLDN-3 expression in these 16HBE cells. HDM-induced cytokine (IL-6, CCL-22, IL-10 and CXCL-8) release was significantly increased after DEP pre-exposure. In the current study an in vitro model with long term PM exposure was presented, which might be helpful for further understanding the interplay between long term PM exposure and allergic responses.",

keywords = "16HBE cells, Air pollution, Airway epithelial cells, Allergy, Chronic, House dust mite, In vitro, Immune responses, Particulate matter",

author = "Hamed Janbazacyabar and \{van Bergenhenegouwen\}, Jeroen and Soheil Varasteh and Johan Garssen and Gert Folkerts and Saskia Braber",

note = "Funding Information: The authors would like to thank Mies van Steenbergen for his technical support related to diesel exhaust particles preparation and size examination. Conflict of Interest: JvB and JG are employees of DNR. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Funding Source: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. Publisher Copyright: {\textcopyright} 2022 The Authors",

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AU - van Bergenhenegouwen, Jeroen

AU - Varasteh, Soheil

AU - Garssen, Johan

AU - Folkerts, Gert

AU - Braber, Saskia

N1 - Funding Information: The authors would like to thank Mies van Steenbergen for his technical support related to diesel exhaust particles preparation and size examination. Conflict of Interest: JvB and JG are employees of DNR. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Funding Source: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. Publisher Copyright: © 2022 The Authors

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N2 - Long term particulate matter (PM) exposure has been associated with an increased incidence of respiratory diseases. Here, an in vitro model was developed to study how long term diesel exhaust particle (DEP) exposure might predispose to the development of allergic reactions. Airway epithelial (16HBE) cells were exposed to low concentrations of diesel exhaust particle (DEP) for 4 days after which they were challenged with house dust mite (HDM) extract (24 h). Compared to acute exposure (24 h), 4 days DEP exposure to 16HBE cells further reduced the transepithelial electrical resistance (TEER) and increased CXCL-8 release. DEP pre-exposure aggravated HDM-induced loss of TEER, increased tracer flux across the barrier and reduced CLDN-3 expression in these 16HBE cells. HDM-induced cytokine (IL-6, CCL-22, IL-10 and CXCL-8) release was significantly increased after DEP pre-exposure. In the current study an in vitro model with long term PM exposure was presented, which might be helpful for further understanding the interplay between long term PM exposure and allergic responses.

AB - Long term particulate matter (PM) exposure has been associated with an increased incidence of respiratory diseases. Here, an in vitro model was developed to study how long term diesel exhaust particle (DEP) exposure might predispose to the development of allergic reactions. Airway epithelial (16HBE) cells were exposed to low concentrations of diesel exhaust particle (DEP) for 4 days after which they were challenged with house dust mite (HDM) extract (24 h). Compared to acute exposure (24 h), 4 days DEP exposure to 16HBE cells further reduced the transepithelial electrical resistance (TEER) and increased CXCL-8 release. DEP pre-exposure aggravated HDM-induced loss of TEER, increased tracer flux across the barrier and reduced CLDN-3 expression in these 16HBE cells. HDM-induced cytokine (IL-6, CCL-22, IL-10 and CXCL-8) release was significantly increased after DEP pre-exposure. In the current study an in vitro model with long term PM exposure was presented, which might be helpful for further understanding the interplay between long term PM exposure and allergic responses.

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KW - Air pollution

KW - Airway epithelial cells

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KW - Chronic

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KW - In vitro, Immune responses

KW - Particulate matter

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Repeated exposure of bronchial epithelial cells to particular matter increases allergen-induced cytokine release and permeability

Abstract

Bibliographical note

Keywords

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