TY - JOUR
T1 - Reductive methylation labeling, from quantitative to structural proteomics
AU - Liu, Z.
AU - Zhou, Ye
AU - Liu, Jing
AU - Chen, Jin
AU - Heck, Albert J.R.
AU - Wang, Fangjun
N1 - Funding Information:
Financial supports are gratefully acknowledged for the National Key R&D Program of China ( 2016YFF0200504 ), the National Natural Science Foundation of China ( 91853101 , 21675152 and 81702995 ) and the grant from DICP ( ZZBS201603 ).
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/9
Y1 - 2019/9
N2 - Reductive methylation is a highly reactive and selective chemical labeling method and plays an important role in proteomic analysis. Various applications of reductive methylation have been developed and have substantially promoted the scope of proteomic analysis to date. Aside from a well-known method for introducing isotopes, this reaction could also be conducted under biological conditions and has little structural perturbations. In this paper, we reviewed the recent developments and applications of reductive methylation in quantitative proteomics and protein N-terminal profiling, along with the emerging applications in structural proteomics for monitoring protein structural/conformational changes under various conditions.
AB - Reductive methylation is a highly reactive and selective chemical labeling method and plays an important role in proteomic analysis. Various applications of reductive methylation have been developed and have substantially promoted the scope of proteomic analysis to date. Aside from a well-known method for introducing isotopes, this reaction could also be conducted under biological conditions and has little structural perturbations. In this paper, we reviewed the recent developments and applications of reductive methylation in quantitative proteomics and protein N-terminal profiling, along with the emerging applications in structural proteomics for monitoring protein structural/conformational changes under various conditions.
KW - Lysine reactivity profiling
KW - Mass spectrometry
KW - N-termini profiling
KW - Quantitative proteomics
KW - Reductive methylation
KW - Stable isotope dimethyl labeling
KW - Structural proteomics
UR - http://www.scopus.com/inward/record.url?scp=85069729282&partnerID=8YFLogxK
U2 - 10.1016/j.trac.2019.07.009
DO - 10.1016/j.trac.2019.07.009
M3 - Review article
AN - SCOPUS:85069729282
SN - 0165-9936
VL - 118
SP - 771
EP - 778
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
ER -