TY - JOUR
T1 - Reduction-sensitive polymer-shell-coated nanogels for intracellular delivery of antigens
AU - Li, Dandan
AU - Chen, Yinan
AU - Mastrobattista, Enrico
AU - Van Nostrum, Cornelus F.
AU - Hennink, Wim E.
AU - Vermonden, Tina
PY - 2017
Y1 - 2017
N2 - Nowadays, layer-by-layer assembled microsized particles receive interest as drug delivery systems. In the present study, we report nanosized hydrogels loaded with a protein antigen that are coated with a disulfide cross-linked polymer shell. These disulfide bonds are stable in the nonreducing extracellular environment, but are reduced in the intracellular environment. This in turn leads to disintegration of the polymer shell and subsequent antigen release. Furthermore, we demonstrate the ability of these core-shell nanogels to boost the MHC class I antigen presentation by dendritic cells to CD8+ T cells.
AB - Nowadays, layer-by-layer assembled microsized particles receive interest as drug delivery systems. In the present study, we report nanosized hydrogels loaded with a protein antigen that are coated with a disulfide cross-linked polymer shell. These disulfide bonds are stable in the nonreducing extracellular environment, but are reduced in the intracellular environment. This in turn leads to disintegration of the polymer shell and subsequent antigen release. Furthermore, we demonstrate the ability of these core-shell nanogels to boost the MHC class I antigen presentation by dendritic cells to CD8+ T cells.
KW - Disulfide
KW - Intracellular delivery
KW - Layer by layer
KW - Nanogel
KW - Ovalbumin
UR - http://www.scopus.com/inward/record.url?scp=85016153474&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.6b00651
DO - 10.1021/acsbiomaterials.6b00651
M3 - Article
AN - SCOPUS:85016153474
SN - 2373-9878
VL - 3
SP - 42
EP - 48
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 1
ER -