Recommendations for Standardizing Thorax PET-CT in Non-Human Primates by Recent Experience from Macaque Studies

Marieke A Stammes; Jaco Bakker; Richard A W Vervenne; Dian G M Zijlmans; Leo van Geest; Michel P M Vierboom; Jan A M Langermans; Frank A W Verreck

doi:10.3390/ani11010204

Recommendations for Standardizing Thorax PET-CT in Non-Human Primates by Recent Experience from Macaque Studies

Marieke A Stammes, Jaco Bakker, Richard A W Vervenne, Dian G M Zijlmans, Leo van Geest, Michel P M Vierboom, Jan A M Langermans, Frank A W Verreck

Department of Parasitology, Biomedical Primate Research Centre, 2288 GJ Rijswijk, The Netherlands.
Animal Science Department, Biomedical Primate Research Centre, 2288 GJ Rijswijk, The Netherlands.

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Despite the possibilities of routine clinical measures and assays on readily accessible bio-samples, it is not always essential in animals to investigate the dynamics of disease longitudinally. In this regard, minimally invasive imaging methods provide powerful tools in preclinical research. They can contribute to the ethical principle of gathering as much relevant information per animal as possible. Besides, with an obvious parallel to clinical diagnostic practice, such imaging platforms are potent and valuable instruments leading to a more refined use of animals from a welfare perspective. Non-human primates comprise highly relevant species for preclinical research to enhance our understanding of disease mechanisms and/or the development of improved prophylactic or therapeutic regimen for various human diseases. In this paper, we describe parameters that critically affect the quality of integrated positron emission tomography and computed tomography (PET-CT) in non-human primates. Lessons learned are exemplified by results from imaging experimental infectious respiratory disease in macaques; specifically tuberculosis, influenza, and SARS-CoV-2 infection. We focus on the thorax and use of 18F-fluorodeoxyglucose as a PET tracer. Recommendations are provided to guide various stages of PET-CT-supported research in non-human primates, from animal selection, scan preparation, and operation, to processing and analysis of imaging data.

Original language	English
Article number	204
Pages (from-to)	1-17
Number of pages	17
Journal	Animals
Volume	11
Issue number	1
DOIs	https://doi.org/10.3390/ani11010204
Publication status	Published - 15 Jan 2021

Bibliographical note

Funding Information:
This research received no external funding. However, the PET?CT infra-structure was supported by a grant from the Bill & Melinda Gates Foundation, OPP1130668. PET?CT NHP procedures optimization was supported in part by the EC Horizon 2020 TRANSVAC2 project, grant agreement no. 730964.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Fluorodeoxyglucose
Macaques
Non-human primates
PET–CT
Respiratory infections
Thorax

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/ani11010204

animals-11-00204-v2Final published version, 4.04 MB

Cite this

@article{19aa34b9965e4a59909fc5c5f25d1322,

title = "Recommendations for Standardizing Thorax PET-CT in Non-Human Primates by Recent Experience from Macaque Studies",

abstract = "Despite the possibilities of routine clinical measures and assays on readily accessible bio-samples, it is not always essential in animals to investigate the dynamics of disease longitudinally. In this regard, minimally invasive imaging methods provide powerful tools in preclinical research. They can contribute to the ethical principle of gathering as much relevant information per animal as possible. Besides, with an obvious parallel to clinical diagnostic practice, such imaging platforms are potent and valuable instruments leading to a more refined use of animals from a welfare perspective. Non-human primates comprise highly relevant species for preclinical research to enhance our understanding of disease mechanisms and/or the development of improved prophylactic or therapeutic regimen for various human diseases. In this paper, we describe parameters that critically affect the quality of integrated positron emission tomography and computed tomography (PET-CT) in non-human primates. Lessons learned are exemplified by results from imaging experimental infectious respiratory disease in macaques; specifically tuberculosis, influenza, and SARS-CoV-2 infection. We focus on the thorax and use of 18F-fluorodeoxyglucose as a PET tracer. Recommendations are provided to guide various stages of PET-CT-supported research in non-human primates, from animal selection, scan preparation, and operation, to processing and analysis of imaging data.",

keywords = "Fluorodeoxyglucose, Macaques, Non-human primates, PET–CT, Respiratory infections, Thorax",

author = "Stammes, {Marieke A} and Jaco Bakker and Vervenne, {Richard A W} and Zijlmans, {Dian G M} and {van Geest}, Leo and Vierboom, {Michel P M} and Langermans, {Jan A M} and Verreck, {Frank A W}",

note = "Funding Information: This research received no external funding. However, the PET?CT infra-structure was supported by a grant from the Bill & Melinda Gates Foundation, OPP1130668. PET?CT NHP procedures optimization was supported in part by the EC Horizon 2020 TRANSVAC2 project, grant agreement no. 730964. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = jan,

day = "15",

doi = "10.3390/ani11010204",

language = "English",

volume = "11",

pages = "1--17",

journal = "Animals",

issn = "2076-2615",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "1",

}

TY - JOUR

T1 - Recommendations for Standardizing Thorax PET-CT in Non-Human Primates by Recent Experience from Macaque Studies

AU - Stammes, Marieke A

AU - Bakker, Jaco

AU - Vervenne, Richard A W

AU - Zijlmans, Dian G M

AU - van Geest, Leo

AU - Vierboom, Michel P M

AU - Langermans, Jan A M

AU - Verreck, Frank A W

N1 - Funding Information: This research received no external funding. However, the PET?CT infra-structure was supported by a grant from the Bill & Melinda Gates Foundation, OPP1130668. PET?CT NHP procedures optimization was supported in part by the EC Horizon 2020 TRANSVAC2 project, grant agreement no. 730964. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/1/15

Y1 - 2021/1/15

N2 - Despite the possibilities of routine clinical measures and assays on readily accessible bio-samples, it is not always essential in animals to investigate the dynamics of disease longitudinally. In this regard, minimally invasive imaging methods provide powerful tools in preclinical research. They can contribute to the ethical principle of gathering as much relevant information per animal as possible. Besides, with an obvious parallel to clinical diagnostic practice, such imaging platforms are potent and valuable instruments leading to a more refined use of animals from a welfare perspective. Non-human primates comprise highly relevant species for preclinical research to enhance our understanding of disease mechanisms and/or the development of improved prophylactic or therapeutic regimen for various human diseases. In this paper, we describe parameters that critically affect the quality of integrated positron emission tomography and computed tomography (PET-CT) in non-human primates. Lessons learned are exemplified by results from imaging experimental infectious respiratory disease in macaques; specifically tuberculosis, influenza, and SARS-CoV-2 infection. We focus on the thorax and use of 18F-fluorodeoxyglucose as a PET tracer. Recommendations are provided to guide various stages of PET-CT-supported research in non-human primates, from animal selection, scan preparation, and operation, to processing and analysis of imaging data.

AB - Despite the possibilities of routine clinical measures and assays on readily accessible bio-samples, it is not always essential in animals to investigate the dynamics of disease longitudinally. In this regard, minimally invasive imaging methods provide powerful tools in preclinical research. They can contribute to the ethical principle of gathering as much relevant information per animal as possible. Besides, with an obvious parallel to clinical diagnostic practice, such imaging platforms are potent and valuable instruments leading to a more refined use of animals from a welfare perspective. Non-human primates comprise highly relevant species for preclinical research to enhance our understanding of disease mechanisms and/or the development of improved prophylactic or therapeutic regimen for various human diseases. In this paper, we describe parameters that critically affect the quality of integrated positron emission tomography and computed tomography (PET-CT) in non-human primates. Lessons learned are exemplified by results from imaging experimental infectious respiratory disease in macaques; specifically tuberculosis, influenza, and SARS-CoV-2 infection. We focus on the thorax and use of 18F-fluorodeoxyglucose as a PET tracer. Recommendations are provided to guide various stages of PET-CT-supported research in non-human primates, from animal selection, scan preparation, and operation, to processing and analysis of imaging data.

KW - Fluorodeoxyglucose

KW - Macaques

KW - Non-human primates

KW - PET–CT

KW - Respiratory infections

KW - Thorax

UR - http://www.scopus.com/inward/record.url?scp=85100060346&partnerID=8YFLogxK

U2 - 10.3390/ani11010204

DO - 10.3390/ani11010204

M3 - Article

C2 - 33467761

SN - 2076-2615

VL - 11

SP - 1

EP - 17

JO - Animals

JF - Animals

IS - 1

M1 - 204

ER -

Recommendations for Standardizing Thorax PET-CT in Non-Human Primates by Recent Experience from Macaque Studies

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this