Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

Glenn R Masson; John E Burke; Natalie G Ahn; Ganesh S Anand; Christoph Borchers; Sébastien Brier; George M Bou-Assaf; John R Engen; S Walter Englander; Johan Faber; Rachel Garlish; Patrick R Griffin; Michael L Gross; Miklos Guttman; Yoshitomo Hamuro; Albert J R Heck; Damian Houde; Roxana E Iacob; Thomas J D Jørgensen; Igor A Kaltashov; Judith P Klinman; Lars Konermann; Petr Man; Leland Mayne; Bruce D Pascal; Dana Reichmann; Mark Skehel; Joost Snijder; Timothy S Strutzenberg; Eric S Underbakke; Cornelia Wagner; Thomas E Wales; Benjamin T Walters; David D Weis; Derek J Wilson; Patrick L Wintrode; Zhongqi Zhang; Jie Zheng; David C Schriemer; Kasper D Rand

doi:10.1038/s41592-019-0459-y

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

Glenn R Masson, John E Burke, Natalie G Ahn, Ganesh S Anand, Christoph Borchers, Sébastien Brier, George M Bou-Assaf, John R Engen, S Walter Englander, Johan Faber, Rachel Garlish, Patrick R Griffin, Michael L Gross, Miklos Guttman, Yoshitomo Hamuro, Albert J R Heck, Damian Houde, Roxana E Iacob, Thomas J D Jørgensen, Igor A KaltashovJudith P Klinman, Lars Konermann, Petr Man, Leland Mayne, Bruce D Pascal, Dana Reichmann, Mark Skehel, Joost Snijder, Timothy S Strutzenberg, Eric S Underbakke, Cornelia Wagner, Thomas E Wales, Benjamin T Walters, David D Weis, Derek J Wilson, Patrick L Wintrode, Zhongqi Zhang, Jie Zheng, David C Schriemer, Kasper D Rand

Research output: Contribution to journal › Review article › peer-review

Abstract

Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.

Original language	English
Pages (from-to)	595-602
Number of pages	8
Journal	Nature Methods
Volume	16
Issue number	7
DOIs	https://doi.org/10.1038/s41592-019-0459-y
Publication status	Published - 27 Jun 2019

Keywords

Data Analysis
Deuterium Exchange Measurement/methods
Hydrogen-Ion Concentration
Mass Spectrometry/methods

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Masson, G. R., Burke, J. E., Ahn, N. G., Anand, G. S., Borchers, C., Brier, S., Bou-Assaf, G. M., Engen, J. R., Englander, S. W., Faber, J., Garlish, R., Griffin, P. R., Gross, M. L., Guttman, M., Hamuro, Y., Heck, A. J. R., Houde, D., Iacob, R. E., Jørgensen, T. J. D., ... Rand, K. D. (2019). Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments. Nature Methods, 16(7), 595-602. https://doi.org/10.1038/s41592-019-0459-y

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abstract = "Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.",

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author = "Masson, {Glenn R} and Burke, {John E} and Ahn, {Natalie G} and Anand, {Ganesh S} and Christoph Borchers and S{\'e}bastien Brier and Bou-Assaf, {George M} and Engen, {John R} and Englander, {S Walter} and Johan Faber and Rachel Garlish and Griffin, {Patrick R} and Gross, {Michael L} and Miklos Guttman and Yoshitomo Hamuro and Heck, {Albert J R} and Damian Houde and Iacob, {Roxana E} and J{\o}rgensen, {Thomas J D} and Kaltashov, {Igor A} and Klinman, {Judith P} and Lars Konermann and Petr Man and Leland Mayne and Pascal, {Bruce D} and Dana Reichmann and Mark Skehel and Joost Snijder and Strutzenberg, {Timothy S} and Underbakke, {Eric S} and Cornelia Wagner and Wales, {Thomas E} and Walters, {Benjamin T} and Weis, {David D} and Wilson, {Derek J} and Wintrode, {Patrick L} and Zhongqi Zhang and Jie Zheng and Schriemer, {David C} and Rand, {Kasper D}",

year = "2019",

month = jun,

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doi = "10.1038/s41592-019-0459-y",

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Masson, GR, Burke, JE, Ahn, NG, Anand, GS, Borchers, C, Brier, S, Bou-Assaf, GM, Engen, JR, Englander, SW, Faber, J, Garlish, R, Griffin, PR, Gross, ML, Guttman, M, Hamuro, Y, Heck, AJR, Houde, D, Iacob, RE, Jørgensen, TJD, Kaltashov, IA, Klinman, JP, Konermann, L, Man, P, Mayne, L, Pascal, BD, Reichmann, D, Skehel, M, Snijder, J, Strutzenberg, TS, Underbakke, ES, Wagner, C, Wales, TE, Walters, BT, Weis, DD, Wilson, DJ, Wintrode, PL, Zhang, Z, Zheng, J, Schriemer, DC & Rand, KD 2019, 'Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments', Nature Methods, vol. 16, no. 7, pp. 595-602. https://doi.org/10.1038/s41592-019-0459-y

TY - JOUR

T1 - Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

AU - Masson, Glenn R

AU - Burke, John E

AU - Ahn, Natalie G

AU - Anand, Ganesh S

AU - Borchers, Christoph

AU - Brier, Sébastien

AU - Bou-Assaf, George M

AU - Engen, John R

AU - Englander, S Walter

AU - Faber, Johan

AU - Garlish, Rachel

AU - Griffin, Patrick R

AU - Gross, Michael L

AU - Guttman, Miklos

AU - Hamuro, Yoshitomo

AU - Heck, Albert J R

AU - Houde, Damian

AU - Iacob, Roxana E

AU - Jørgensen, Thomas J D

AU - Kaltashov, Igor A

AU - Klinman, Judith P

AU - Konermann, Lars

AU - Man, Petr

AU - Mayne, Leland

AU - Pascal, Bruce D

AU - Reichmann, Dana

AU - Skehel, Mark

AU - Snijder, Joost

AU - Strutzenberg, Timothy S

AU - Underbakke, Eric S

AU - Wagner, Cornelia

AU - Wales, Thomas E

AU - Walters, Benjamin T

AU - Weis, David D

AU - Wilson, Derek J

AU - Wintrode, Patrick L

AU - Zhang, Zhongqi

AU - Zheng, Jie

AU - Schriemer, David C

AU - Rand, Kasper D

PY - 2019/6/27

Y1 - 2019/6/27

N2 - Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.

AB - Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful biophysical technique being increasingly applied to a wide variety of problems. As the HDX-MS community continues to grow, adoption of best practices in data collection, analysis, presentation and interpretation will greatly enhance the accessibility of this technique to nonspecialists. Here we provide recommendations arising from community discussions emerging out of the first International Conference on Hydrogen-Exchange Mass Spectrometry (IC-HDX; 2017). It is meant to represent both a consensus viewpoint and an opportunity to stimulate further additions and refinements as the field advances.

KW - Data Analysis

KW - Deuterium Exchange Measurement/methods

KW - Hydrogen-Ion Concentration

KW - Mass Spectrometry/methods

U2 - 10.1038/s41592-019-0459-y

DO - 10.1038/s41592-019-0459-y

M3 - Review article

C2 - 31249422

SN - 1548-7091

VL - 16

SP - 595

EP - 602

JO - Nature Methods

JF - Nature Methods

IS - 7

ER -

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

Abstract

Keywords

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