TY - JOUR
T1 - Real-time PCR detection of ruminant DNA
AU - Mendoza-Romero, Luis
AU - Verkaar, Edward L C
AU - Savelkoul, Paul H.
AU - Catsburg, Arnold
AU - Aarts, Henk J M
AU - Buntjer, Jaap B.
AU - Lenstra, Johannes A.
PY - 2004/3/1
Y1 - 2004/3/1
N2 - To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.
AB - To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.
KW - Diergeneeskunde (DGNK)
UR - http://www.scopus.com/inward/record.url?scp=2142774012&partnerID=8YFLogxK
M3 - Article
SN - 0362-028X
VL - 67
SP - 550
EP - 554
JO - Journal of Food Protection
JF - Journal of Food Protection
IS - 3
ER -