Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY(58l/591)

E.H.W. Pap; G.P.C. Drummen; V.J. Winter; T.W.A. Kooij; P. Rijken; K.W.A. Wirtz; J.A.F. Op Den Kamp; W.J. Hage; J.A. Post

doi:10.1016/S0014-5793(99)00696-1

Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY(58l/591)

E.H.W. Pap
, G.P.C. Drummen
, V.J. Winter
, T.W.A. Kooij
, P. Rijken
, K.W.A. Wirtz
, J.A.F. Op Den Kamp
, W.J. Hage
, J.A. Post

Cell Biology, Neurobiology and Biophysics

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A ratio-fluorescence assay was developed for on-line localization and quantification of lipid oxidation in living cells. The assay explores the oxidative sensitivity of C11-BODIPY(581/591). Upon oxidation, the fluorescence of this fluorophore shifts from red to green. The probe incorporates readily into cellular membranes and is about twice as sensitive to oxidation as arachidonic acid. Using confocal microscopy, the cumene hydroperoxide-induced oxidation of C11-BODIPY(581/591) was visualized at the sub-cellular level in rat-1 fibroblasts. Preloading of the cells with tocopherol retarded this oxidation. The data demonstrate that C11-BODIPY(581/591) is a valuable tool to quantify lipid oxidation and anti-oxidant efficacy in single cells.

Original language	English
Pages (from-to)	278-282
Number of pages	5
Journal	FEBS Letters
Volume	453
Issue number	3
DOIs	https://doi.org/10.1016/S0014-5793(99)00696-1
Publication status	Published - 25 Jun 1999

Keywords

BODIPY
Lipid oxidation
Oxygen radical
Ratio-fluorescence microscopy
Tocopherol
cumene
tocopherol
animal cell
article
confocal microscopy
fibroblast
fluorescence microscopy
lipid oxidation
nonhuman
priority journal
rat

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10.1016/S0014-5793(99)00696-1

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title = "Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY(58l/591)",

abstract = "A ratio-fluorescence assay was developed for on-line localization and quantification of lipid oxidation in living cells. The assay explores the oxidative sensitivity of C11-BODIPY(581/591). Upon oxidation, the fluorescence of this fluorophore shifts from red to green. The probe incorporates readily into cellular membranes and is about twice as sensitive to oxidation as arachidonic acid. Using confocal microscopy, the cumene hydroperoxide-induced oxidation of C11-BODIPY(581/591) was visualized at the sub-cellular level in rat-1 fibroblasts. Preloading of the cells with tocopherol retarded this oxidation. The data demonstrate that C11-BODIPY(581/591) is a valuable tool to quantify lipid oxidation and anti-oxidant efficacy in single cells.",

keywords = "BODIPY, Lipid oxidation, Oxygen radical, Ratio-fluorescence microscopy, Tocopherol, cumene, tocopherol, animal cell, article, confocal microscopy, fibroblast, fluorescence microscopy, lipid oxidation, nonhuman, priority journal, rat",

author = "E.H.W. Pap and G.P.C. Drummen and V.J. Winter and T.W.A. Kooij and P. Rijken and K.W.A. Wirtz and \{Op Den Kamp\}, J.A.F. and W.J. Hage and J.A. Post",

year = "1999",

month = jun,

day = "25",

doi = "10.1016/S0014-5793(99)00696-1",

language = "English",

volume = "453",

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journal = "FEBS Letters",

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T1 - Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY(58l/591)

AU - Pap, E.H.W.

AU - Drummen, G.P.C.

AU - Winter, V.J.

AU - Kooij, T.W.A.

AU - Rijken, P.

AU - Wirtz, K.W.A.

AU - Op Den Kamp, J.A.F.

AU - Hage, W.J.

AU - Post, J.A.

PY - 1999/6/25

Y1 - 1999/6/25

N2 - A ratio-fluorescence assay was developed for on-line localization and quantification of lipid oxidation in living cells. The assay explores the oxidative sensitivity of C11-BODIPY(581/591). Upon oxidation, the fluorescence of this fluorophore shifts from red to green. The probe incorporates readily into cellular membranes and is about twice as sensitive to oxidation as arachidonic acid. Using confocal microscopy, the cumene hydroperoxide-induced oxidation of C11-BODIPY(581/591) was visualized at the sub-cellular level in rat-1 fibroblasts. Preloading of the cells with tocopherol retarded this oxidation. The data demonstrate that C11-BODIPY(581/591) is a valuable tool to quantify lipid oxidation and anti-oxidant efficacy in single cells.

AB - A ratio-fluorescence assay was developed for on-line localization and quantification of lipid oxidation in living cells. The assay explores the oxidative sensitivity of C11-BODIPY(581/591). Upon oxidation, the fluorescence of this fluorophore shifts from red to green. The probe incorporates readily into cellular membranes and is about twice as sensitive to oxidation as arachidonic acid. Using confocal microscopy, the cumene hydroperoxide-induced oxidation of C11-BODIPY(581/591) was visualized at the sub-cellular level in rat-1 fibroblasts. Preloading of the cells with tocopherol retarded this oxidation. The data demonstrate that C11-BODIPY(581/591) is a valuable tool to quantify lipid oxidation and anti-oxidant efficacy in single cells.

KW - BODIPY

KW - Lipid oxidation

KW - Oxygen radical

KW - Ratio-fluorescence microscopy

KW - Tocopherol

KW - cumene

KW - tocopherol

KW - animal cell

KW - article

KW - confocal microscopy

KW - fibroblast

KW - fluorescence microscopy

KW - lipid oxidation

KW - nonhuman

KW - priority journal

KW - rat

U2 - 10.1016/S0014-5793(99)00696-1

DO - 10.1016/S0014-5793(99)00696-1

M3 - Article

SN - 0014-5793

VL - 453

SP - 278

EP - 282

JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY(58l/591)

Abstract

Keywords

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