TY - JOUR
T1 - Rapid species identification in meat by using satellite DNA probes
AU - Buntjer, Jacob B.
AU - Lenstra, Johannes A.
AU - Haagsma, Nel
PY - 1995/11/1
Y1 - 1995/11/1
N2 - A fast procedure for species identification in heated meat is described. Deoxyribonucleic acid (DNA) was isolated from meat samples by an alkaline extraction method and hybridized to a conjugate of a specific oligonucleotide and alkaline phosphatase. The oligonucleotide probes are based on satellite DNA, tandem-repeated sequences, which are highly specific for species. Probes are developed for the identification of meat from cattle, sheep/goat, horse, deer, pig, chicken and turkey. Differentiation between closely related species like chicken and turkey is possible. Admixtures of 1-5% of meat of one species in another could be detected. The complete assay of up to 50 samples takes 4 h. Heated meat samples could be analysed.
AB - A fast procedure for species identification in heated meat is described. Deoxyribonucleic acid (DNA) was isolated from meat samples by an alkaline extraction method and hybridized to a conjugate of a specific oligonucleotide and alkaline phosphatase. The oligonucleotide probes are based on satellite DNA, tandem-repeated sequences, which are highly specific for species. Probes are developed for the identification of meat from cattle, sheep/goat, horse, deer, pig, chicken and turkey. Differentiation between closely related species like chicken and turkey is possible. Admixtures of 1-5% of meat of one species in another could be detected. The complete assay of up to 50 samples takes 4 h. Heated meat samples could be analysed.
UR - http://www.scopus.com/inward/record.url?scp=0029442375&partnerID=8YFLogxK
U2 - 10.1007/BF01201589
DO - 10.1007/BF01201589
M3 - Article
C2 - 8585335
AN - SCOPUS:0029442375
SN - 0044-3026
VL - 201
SP - 577
EP - 582
JO - Zeitschrift für Lebensmittel-Untersuchung und Forschung
JF - Zeitschrift für Lebensmittel-Untersuchung und Forschung
IS - 6
ER -