Rapid screening of lectins for multivalency effects with a glycodendrimer microarray

Núria Parera Pera; Hilbert M. Branderhorst; Raymond Kooij; Caroline Maierhofer; Marjolein Van Der Kaaden; Rob M. J. Liskamp; Valentin Wittmann; Rob Ruijtenbeek; Roland J. Pieters

doi:10.1002/cbic.201000340

Rapid screening of lectins for multivalency effects with a glycodendrimer microarray

Núria Parera Pera, Hilbert M. Branderhorst, Raymond Kooij, Caroline Maierhofer, Marjolein Van Der Kaaden, Rob M. J. Liskamp, Valentin Wittmann, Rob Ruijtenbeek, Roland J. Pieters

Dept of Pharmaceutical Sciences

Universität Konstanz

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Multivalency is an important phenomenon in protein-carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Original language	English
Pages (from-to)	1896-1904
Number of pages	9
Journal	ChemBioChem
Volume	11
Issue number	13
DOIs	https://doi.org/10.1002/cbic.201000340
Publication status	Published - 3 Sept 2010

Keywords

Carbohydrates
Click chemistry
Dendrimers
Inhibitors
Microarrays
carbohydrate
dendrimer
fluorescent dye
lectin
n acetylglucosamine
sugar
article
binding kinetics
microarray analysis
monitoring
priority journal
protein binding
protein carbohydrate interaction
screening
synthesis

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title = "Rapid screening of lectins for multivalency effects with a glycodendrimer microarray",

abstract = "Multivalency is an important phenomenon in protein-carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins. {\textcopyright} 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.",

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author = "{Parera Pera}, N{\'u}ria and Branderhorst, {Hilbert M.} and Raymond Kooij and Caroline Maierhofer and {Van Der Kaaden}, Marjolein and Liskamp, {Rob M. J.} and Valentin Wittmann and Rob Ruijtenbeek and Pieters, {Roland J.}",

year = "2010",

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doi = "10.1002/cbic.201000340",

language = "English",

volume = "11",

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AU - Parera Pera, Núria

AU - Branderhorst, Hilbert M.

AU - Kooij, Raymond

AU - Maierhofer, Caroline

AU - Van Der Kaaden, Marjolein

AU - Liskamp, Rob M. J.

AU - Wittmann, Valentin

AU - Ruijtenbeek, Rob

AU - Pieters, Roland J.

PY - 2010/9/3

Y1 - 2010/9/3

N2 - Multivalency is an important phenomenon in protein-carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - Multivalency is an important phenomenon in protein-carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

KW - Carbohydrates

KW - Click chemistry

KW - Dendrimers

KW - Inhibitors

KW - Microarrays

KW - carbohydrate

KW - dendrimer

KW - fluorescent dye

KW - lectin

KW - n acetylglucosamine

KW - sugar

KW - article

KW - binding kinetics

KW - microarray analysis

KW - monitoring

KW - priority journal

KW - protein binding

KW - protein carbohydrate interaction

KW - screening

KW - synthesis

U2 - 10.1002/cbic.201000340

DO - 10.1002/cbic.201000340

M3 - Article

SN - 1439-4227

VL - 11

SP - 1896

EP - 1904

JO - ChemBioChem

JF - ChemBioChem

IS - 13

ER -

Rapid screening of lectins for multivalency effects with a glycodendrimer microarray

Abstract

Keywords

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