Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Marc Stegger; Jodi A Lindsay; Arshnee Moodley; Robert Skov; Els M Broens; Luca Guardabassi

doi:10.1128/JCM.01970-10

Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Marc Stegger, Jodi A Lindsay, Arshnee Moodley, Robert Skov, Els M Broens, Luca Guardabassi

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.

Original language	English
Pages (from-to)	732-4
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	2
DOIs	https://doi.org/10.1128/JCM.01970-10
Publication status	Published - Feb 2011

Keywords

Animals
Bacterial Typing Techniques
DNA Restriction-Modification Enzymes
Humans
Polymerase Chain Reaction
Sensitivity and Specificity
Staphylococcal Infections
Staphylococcus aureus

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10.1128/JCM.01970-10

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title = "Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1",

abstract = "A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.",

keywords = "Animals, Bacterial Typing Techniques, DNA Restriction-Modification Enzymes, Humans, Polymerase Chain Reaction, Sensitivity and Specificity, Staphylococcal Infections, Staphylococcus aureus",

author = "Marc Stegger and Lindsay, {Jodi A} and Arshnee Moodley and Robert Skov and Broens, {Els M} and Luca Guardabassi",

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AU - Stegger, Marc

AU - Lindsay, Jodi A

AU - Moodley, Arshnee

AU - Skov, Robert

AU - Broens, Els M

AU - Guardabassi, Luca

PY - 2011/2

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AB - A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection (n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.

KW - Animals

KW - Bacterial Typing Techniques

KW - DNA Restriction-Modification Enzymes

KW - Humans

KW - Polymerase Chain Reaction

KW - Sensitivity and Specificity

KW - Staphylococcal Infections

KW - Staphylococcus aureus

U2 - 10.1128/JCM.01970-10

DO - 10.1128/JCM.01970-10

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -

Rapid PCR detection of Staphylococcus aureus clonal complex 398 by targeting the restriction-modification system carrying sau1-hsdS1

Abstract

Keywords

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