Abstract
The construction of new nanotools is presented here using the example of fluorescent semiconductor nanocrystals, quantum dots (QDs). In this study, the implementation of the new lipid oligonucleotide conjugate-functionalized quantum dots (LON-QDs) is realized in four steps: (i) the synthesis of the lipid oligonucleotide conjugates (LONs), (ii) the encapsulation of QDs by nucleolipids and LONs, (iii) the study of the duplex formation of LON-QDs with the complementary ON partners, and (iv) the cellular uptake of the LON-QD platform and hybridization with the target ONs (microRNA and miR-21).
Original language | English |
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Pages (from-to) | 1345-55 |
Number of pages | 11 |
Journal | Bioconjugate Chemistry |
Volume | 24 |
Issue number | 8 |
DOIs | |
Publication status | Published - 21 Aug 2013 |
Keywords
- Base Sequence
- Biological Transport
- HeLa Cells
- Humans
- Hydrophobic and Hydrophilic Interactions
- Intracellular Space
- Kinetics
- Lipids
- MicroRNAs
- Nucleic Acid Hybridization
- Oligonucleotides
- Quantum Dots
- Semiconductors
- Solubility
- Journal Article
- Research Support, Non-U.S. Gov't