Quantum dot lipid oligonucleotide bioconjugates: toward a new anti-microRNA nanoplatform

Ahissan Aimé, Nataliia Beztsinna, Amit Patwa, Alexander Pokolenko, Isabelle Bestel, Philippe Barthélémy

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The construction of new nanotools is presented here using the example of fluorescent semiconductor nanocrystals, quantum dots (QDs). In this study, the implementation of the new lipid oligonucleotide conjugate-functionalized quantum dots (LON-QDs) is realized in four steps: (i) the synthesis of the lipid oligonucleotide conjugates (LONs), (ii) the encapsulation of QDs by nucleolipids and LONs, (iii) the study of the duplex formation of LON-QDs with the complementary ON partners, and (iv) the cellular uptake of the LON-QD platform and hybridization with the target ONs (microRNA and miR-21).

Original languageEnglish
Pages (from-to)1345-55
Number of pages11
JournalBioconjugate Chemistry
Volume24
Issue number8
DOIs
Publication statusPublished - 21 Aug 2013

Keywords

  • Base Sequence
  • Biological Transport
  • HeLa Cells
  • Humans
  • Hydrophobic and Hydrophilic Interactions
  • Intracellular Space
  • Kinetics
  • Lipids
  • MicroRNAs
  • Nucleic Acid Hybridization
  • Oligonucleotides
  • Quantum Dots
  • Semiconductors
  • Solubility
  • Journal Article
  • Research Support, Non-U.S. Gov't

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