Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster Resonance Energy Transfer

T. Skajaa; Y. Zhao; D.J. van den Heuvel; H.C. Gerritsen; D.P. Cormode; R. Koole; M.M. van Schooneveld; J.A. Post; E.A. Fisher; Z.A. Fayad; C. de Mello Donega; A. Meijerink; W.J.M. Mulder

doi:10.1021/nl1037903

Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster Resonance Energy Transfer

T. Skajaa, Y. Zhao, D.J. van den Heuvel, H.C. Gerritsen, D.P. Cormode, R. Koole, M.M. van Schooneveld, J.A. Post, E.A. Fisher, Z.A. Fayad, C. de Mello Donega, A. Meijerink, W.J.M. Mulder

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Abstract

The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Frster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein−lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.

Original language	English
Pages (from-to)	5131-5138
Number of pages	8
Journal	Nano Letters
Volume	10
DOIs	https://doi.org/10.1021/nl1037903
Publication status	Published - 2010

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Skajaa, T., Zhao, Y., van den Heuvel, D. J., Gerritsen, H. C., Cormode, D. P., Koole, R., van Schooneveld, M. M., Post, J. A., Fisher, E. A., Fayad, Z. A., de Mello Donega, C., Meijerink, A., & Mulder, W. J. M. (2010). Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster Resonance Energy Transfer. Nano Letters, 10, 5131-5138. https://doi.org/10.1021/nl1037903

@article{5587072231cd4932baabda3e530fa0f2,

title = "Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via F{\"o}rster Resonance Energy Transfer",

abstract = "The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Frster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein−lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.",

author = "T. Skajaa and Y. Zhao and {van den Heuvel}, D.J. and H.C. Gerritsen and D.P. Cormode and R. Koole and {van Schooneveld}, M.M. and J.A. Post and E.A. Fisher and Z.A. Fayad and {de Mello Donega}, C. and A. Meijerink and W.J.M. Mulder",

year = "2010",

doi = "10.1021/nl1037903",

language = "English",

volume = "10",

pages = "5131--5138",

journal = "Nano Letters",

issn = "1530-6984",

publisher = "American Chemical Society",

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Skajaa, T, Zhao, Y, van den Heuvel, DJ , Gerritsen, HC, Cormode, DP, Koole, R, van Schooneveld, MM, Post, JA, Fisher, EA, Fayad, ZA, de Mello Donega, C , Meijerink, A & Mulder, WJM 2010, 'Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster Resonance Energy Transfer', Nano Letters, vol. 10, pp. 5131-5138. https://doi.org/10.1021/nl1037903

TY - JOUR

T1 - Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster Resonance Energy Transfer

AU - Skajaa, T.

AU - Zhao, Y.

AU - van den Heuvel, D.J.

AU - Gerritsen, H.C.

AU - Cormode, D.P.

AU - Koole, R.

AU - van Schooneveld, M.M.

AU - Post, J.A.

AU - Fisher, E.A.

AU - Fayad, Z.A.

AU - de Mello Donega, C.

AU - Meijerink, A.

AU - Mulder, W.J.M.

PY - 2010

Y1 - 2010

N2 - The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Frster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein−lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.

AB - The study of lipoproteins, natural nanoparticles comprised of lipids and apolipoproteins that transport fats throughout the body, is of key importance to better understand, treat, and prevent cardiovascular disease. In the current study, we have developed a lipoprotein-based nanoparticle that consists of a quantum dot (QD) core and Cy5.5 labeled lipidic coating. The methodology allows judicious tuning of the QD/Cy5.5 ratio, which enabled us to optimize Frster resonance energy transfer (FRET) between the QD core and the Cy5.5-labeled coating. This phenomenon allowed us to study lipoprotein−lipoprotein interactions, lipid exchange dynamics, and the influence of apolipoproteins on these processes. Moreover, we were able to study HDL-cell interactions and exploit FRET to visualize HDL association with live macrophage cells.

U2 - 10.1021/nl1037903

DO - 10.1021/nl1037903

M3 - Article

SN - 1530-6984

VL - 10

SP - 5131

EP - 5138

JO - Nano Letters

JF - Nano Letters

ER -

Quantum dot and Cy5.5 labeled nanoparticles to investigate lipoprotein biointeractions via Förster Resonance Energy Transfer

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