Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

Annemarie Nadort, Rutger G. Woolthuis, Ton G. van Leeuwen, Dirk J. Faber

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, noninvasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDFLSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources. © 2013 Optical Society of America.
Original languageEnglish
Pages (from-to)2347-2361
Number of pages15
JournalBiomedical Optics Express
Volume4
Issue number11
DOIs
Publication statusPublished - 1 Nov 2013

Keywords

  • glass fiber
  • hemoglobin
  • intralipid
  • silastic
  • titanium dioxide
  • article
  • density functional theory
  • electric field
  • flow rate
  • human
  • human experiment
  • instrument validation
  • laser
  • laser Doppler flowmetry
  • microcirculation
  • normal human
  • optical coherence tomography
  • phantom
  • photon
  • sidestream dark field flowmetry
  • spatial analysis
  • sublingual gland

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